山东科学2013，Vol.26Issue(1)：47-50,64,5.DOI:10.3976/j.issn.1002-4026.2013.01.010
不同加工方法杜梨叶中鞣质的含量测定
Determination of tannin content in leaves of Pyrus betulaefolia Bge.with different processing methods
王瑞华 1侯彩婷 1邹昀员 1田易玲 1刘玉红 1徐凌川1
作者信息
- 1. 山东中医药大学药学院,山东济南250355
- 折叠
摘要
Abstract
We employ phosphorus molybdenumtungsten acid casein method to determine tannin content of two different products from the leaves of Pyrus betulaefolia Bge.. Tannins were extracted to be soaking at room temperature by staying overnight When the color reaction between phosphomolybdium Tungslic acid and phenolic acids was carrying through 90～120 min,absorbency of tolal phenolic acids and unabsorbable phenolic acids was determined respectively at The wavelength of 760 nm. Tannins content is derived by spectrophotometry difference and standard curve method. Better linear relationship (r=0.997 9) exists in the range of 1～10 μg/mL. We obtain average recovery rate of 98.581% (n = 6) and RSD of 0.51%. Tannins average content of unfermented leaves is 0.60%, and tannins is not detected in the leaves of fermentation under this condition.关键词
杜梨叶/磷钼钨酸-干酪素法/鞣质/含量测定Key words
Pyrus betulaefolia Bge. / phosphorus molybdenum-tungsten acid casein/ tannin/ content determination分类
医药卫生引用本文复制引用
王瑞华,侯彩婷,邹昀员,田易玲,刘玉红,徐凌川..不同加工方法杜梨叶中鞣质的含量测定[J].山东科学,2013,26(1):47-50,64,5.
