山东中医药大学学报2013，Vol.37Issue(1)：73-76,4.
关黄柏不同炮制品中盐酸小檗碱与盐酸巴马汀含量测定
Determination of berberine hydrochloride and palmatine hydrochloride in different processing products of phellodendri amurensis cortex
摘要
Abstract
Objective:Taking berberine hydrochloride and palmatine hydrochloride content as indicators to study the impact of different processing methods on the phellodendri amurensis cortex quaternary ammonium bases ingredients. Methods:We use HPLC method for the determination of different processing products of berberine hydrochloride and palmatine hydrochloride content. The column is performed on a Diamonsil TM(Diamond) C18 column(250mm×4.6mm,5 μm),the mobile phase of ace-tonitrile is 0.1% phosphoric acid(0.02mol·L1 sodium dihydrogen phosphate)(25:75),the flow rate is 1.0 mL·min-1,and column temperature 25℃;detection wavelength is 345 nm and reference wavelength is 500 run. Results:The content of the different processing products of berberine hydrochloride and palmatine hydrochloride in descending order is as follows:wine fried pro-duts>raw products>salt fried products>fried carbon products,there is no significant difference between raw products and salt fried products,fried carbon only detected a small amount of the product. Conclusion:As the processing extent increased,berberine hydrochloride and palmatine hydrochloride in the fried carbon products dropped significantly,while the wine fried produts of both increased significantly,it is worth further study.关键词
关黄柏/炮制品/盐酸小檗碱/盐酸巴马汀/HPLCKey words
phellodendri amurensis cortex/processing products/berberine hydrochloride/palmatine hydrochloride/HPLC分类
医药卫生引用本文复制引用
张超..关黄柏不同炮制品中盐酸小檗碱与盐酸巴马汀含量测定[J].山东中医药大学学报,2013,37(1):73-76,4.基金项目
"十一五"重大新药创制项目(编号:2009ZX09301-013) （编号:2009ZX09301-013）
