山东农业科学2012，Vol.44Issue(11)：4-7,4.
番茄黄化曲叶病毒山东泰安分离物的全基因组克隆与分析
Cloning and Sequence Analysis of Taian Isolate of Tomato Yellow Leaf Curl Virus in Shandong Province
摘要
Abstract
The samples ( SDTA) showing typical symptoms of tomato yellow leaf curl disease were collected from protected tomato planting field of Taian, Shandong Province in spring of 2011. In order to identify the causal virus, the universal primers and the primers for full - length DNA - A gene amplification of gemini-virus were used to amplify the sample, and a 2 781 bp fragment of the virus complete genome was obtained. The sequence analysis showed the complete genomic sequence of SDTA had the highest identity of 99. 6% with tomato yellow leaf curl virus isolate of Anhui ( FN650807) , and the phylogenic analysis results indicated that SDTA belonged to Israel strain of the tomato yellow leaf curl virus.关键词
番茄黄化曲叶病毒/DNA-A/序列分析Key words
Tomato yellow leaf curl virus/ DNA-A/ Sequence analysis分类
生物科学引用本文复制引用
刘文浩,仇平平,辛相启,苏文敏,王祥,竺晓平..番茄黄化曲叶病毒山东泰安分离物的全基因组克隆与分析[J].山东农业科学,2012,44(11):4-7,4.基金项目
国家公益性行业科研专项(201003065)资助. （201003065）
