山东农业科学2012，Vol.44Issue(11)：112-116,5.
石榴果实酚类物质测定体系优化与不同部位组分及含量测定
Optimization of Detection System for Polyphenol and Its Compositions and Contents in Different Parts of Pomegranate Fruit
摘要
Abstract
A high performance liquid chromatographic method was developed for the analysis of polyphenol compositions and contents in different parts of ' Taishanhong' pomegranate fruit including peel, seed and juice. The chromatographic separation was performed on a Kromasil (250 mm×4. 6 mm, 5μm). The mobile phase was acetonitrile and 1% acetate acid solution for gradient elution. The column temperature was 30℃; the flow rate was 1. 1 ml/min and the wave length was 280 nm. The results indicated that thirteen phenolic compounds were identified in pomegranate peel and seed, including gallic acid, chlorogenic acid, para-hydroxybenzoic acid, epicatechin, caffeic acid, catechin, vanillin, ferulic acid, benzoic acid, phloridzin, quercetin, cinnamic acid and phloretin. Twelve phenolic compounds were identified in pomegranate juice, and epicatechin was not detected. The content of polyphenols was the highest in pomegranate peel, followed by pomegranate juice and pomegranate seed. The major acidic phenolic compound and flavonoid compound in pomegranate peel were parahydroxybenzoic acid (0.828 mg/g) and epicatechin (0.915 mg/g) respectively, while those in pomegranate juice were parahydroxybenzoic acid (0. 12 mg/g) and catechin (0. 149 mg/g) respectively, and those in pomegranate seed were caffeic acid (0.026 mg/g) and phloridzin (0.075 mg/g) respectively.关键词
石榴/多酚/高效液相色谱Key words
Pomegranate/ Polyphenol/ HPLC分类
农业科技引用本文复制引用
韩玲玲,苑兆和,冯立娟,杨尚尚,朱峰..石榴果实酚类物质测定体系优化与不同部位组分及含量测定[J].山东农业科学,2012,44(11):112-116,5.基金项目
山东省科技发展计划项目(2011GNC11025) （2011GNC11025）
