山东农业科学2013，Vol.45Issue(1)：9-13,5.
花生蛋白激酶基因AhSTK的克隆与序列分析
Cloning and Sequence Analysis of Protein Kinase Gene AhSTK from Arachis hypogaea L.
摘要
Abstract
In this study, one new sequence total as 1 399 bp with polyA in 3' end was obtained from the cDNA library of peanut cultivar Luhua 14. By blastx analysis, it was identified as a protein kinase gene, named as AhSTK. With its cDNA sequence as template, the specific primers were designed to clone the AhSTK gene. The sequence analysis indicated that the open reading frame of AhSTK gene had 1 080 bp bases, and encoding 359 amino acids with the molecular weight about 38.9 kD and the isoelectric point about 6.42. The encoded protein had no signal peptide and transmembrane domains, and was predicted to localize in cytoplasm. Compared with the amino acid sequences of other plant protein kinases, AhSTK had the highest homol-ogy with that of soybean. Organ specificity analysis showed that its expression level was higher in peanut roots, but lower in flowers. The cloning and sequence analysis of AhSTK gene would lay foundations for further study of its biological functions and practical application.关键词
花生/蛋白激酶/AhSTK蛋白/序列分析/器官特异性表达Key words
Peanut/ Protein kinase/ AhSTK protein/ Sequence analysis/ Organ specific expression分类
生物科学引用本文复制引用
朱岩,彭振英,范仲学,张斌,王成祥,郑玲,毕玉平..花生蛋白激酶基因AhSTK的克隆与序列分析[J].山东农业科学,2013,45(1):9-13,5.基金项目
国家自然科学基金(30871541),国家国际科技合作专项项目(2012DFA30450),山东省济南市科技发展计划项目(201004044) （30871541）
