山东农业科学2013，Vol.45Issue(1)：96-99,4.
嗜麦芽窄食单胞菌YHYJ-1角蛋白酶底物特性及酶活测定方法研究
Study on Substrate Characteristics and Activity Determination Method of Keratinase from Stenotrophomonas maltophilia Strain YHYJ-1
摘要
Abstract
A Stenotrophomonas maltophilia strain YHYJ - 1 isolated from sludge of feather processing plant showed strong degrading capability on feather, wool and human hair. Feather and wool could be degraded completely in 36 h and 72 h respectively, but only part of human hair could be degraded in 84 hours after incubated with YHYJ - 1. When co - incubated with substrates of soluble feather keratin, feather powder and azokeratin, the relative activity of keratinase were 100% , 65.1% and 23.2% , respectively, which indicated that soluble keratin was the optimal substrate. If incubated with feather meal, the keratinase activity increased sharply after incubated for 20 hours, and reached the highest after 28 hours, and then it decreased rapidly.关键词
嗜麦芽窄食单胞菌/羽毛/角蛋白质/角蛋白酶Key words
Stenotrophomonas maltophilia/ Feather/ Keratin/ Keratinase分类
生物科学引用本文复制引用
朱友峰,游银伟,尤升波,邹晓凤,王翠萍,岳寿松..嗜麦芽窄食单胞菌YHYJ-1角蛋白酶底物特性及酶活测定方法研究[J].山东农业科学,2013,45(1):96-99,4.基金项目
山东省科技发展计划(2010GNC10950)资助. （2010GNC10950）
