山东农业科学2013，Vol.45Issue(2)：5-10,6.
小鼠MCK基因启动子的克隆及其活性初步分析
Cloning and Preliminary Activity Analysis of Mouse Muscle Creatine Kinase (MCK) Gene Promoter
摘要
Abstract
The 5' - flanking region and promoter core sequences of mouse muscle creatine kinase (MCK) gene were cloned, and its transcriptional regulatory mechanisms were studied, too. Firstly, the 5' -flanking region of MCK gene was cloned from the mouse tail muscle genomic DNA by PCR, and was jointed into the pEASY - T3 cloning vector after purification. The sequence of enhancer and core promoter were analyzed. Then the core promoter of MCK gene was subcloned into the expression vector pGL3 - MCK2 - Venus. After pGL3 - MCK2 - Venus was transfected into C2C12 cells mediated by LipofectamineTM 2000, its expression was observed with fluorescence microscopy. The results showed that the transcription of MCK was regulated by a core promoter ( - 1354 to + 1) , and the fusion protein could be expressed efficiently in C2C12 cell after transfection. This study confirmed that the core promoter of MCK gene had the ability of organization specificity regulation.关键词
小鼠/MCK基因/启动子/序列分析Key words
Mouse/ MCK gene/ Promoter/ Sequence analysis分类
生物科学引用本文复制引用
赵贵民,王洪梅,冯敏燕,何洪彬..小鼠MCK基因启动子的克隆及其活性初步分析[J].山东农业科学,2013,45(2):5-10,6.基金项目
泰山学者海外特聘专家项目 （）
国家奶牛专业技术体系建设专项 （）
国家转基因重大专项(2009ZX08007-006B,2011ZX08007-002,2011ZX08008-004) （2009ZX08007-006B,2011ZX08007-002,2011ZX08008-004）
山东省自然科学基金项目 （）
