山东农业科学2013，Vol.45Issue(2)：135-138,4.
锌指核酸酶技术在动物转基因研究中的应用
Application of Zinc Finger Nuclease in Animal Transgenic Research
摘要
Abstract
Zinc finger nuclease (ZFN) is a kind of artificial synthesis containing zinc finger protein and the shear structure domain of Fok I endonuclease. Because it can specifically identify and cleave DNA sequences and easily be designed, it is used for the site - directed genetic mutations and integration of exogenous genes. This technology has been applied to the research of transgenic animals. In this paper, the research progress of zinc finger nuclease technology and its application were reviewed, and its application prospect was also forecasted.关键词
锌指蛋白/锌指核酸酶/基因打靶/同源重组/转基因动物Key words
Zinc finger protein/ Zinc finger nuclease/ Gene targeting/ Homologous recombination/ Transgenic animal分类
生物科学引用本文复制引用
刘晓,方永志,刘文浩,王洪梅,何洪彬..锌指核酸酶技术在动物转基因研究中的应用[J].山东农业科学,2013,45(2):135-138,4.基金项目
泰山学者海外特聘专家(何洪彬),国家奶牛产业技术体系建设专项经费(何洪彬),山东省科技攻关课题(2009GG20002032),国家转基因重大专项(2009ZX08007-006B,2011ZX08007-002,2011ZX08008-004),济南市高校院所自主创新计划(201004027). （何洪彬）
