山东医药2013，Vol.53Issue(1)：1-4,4.DOI:10.3969/j.issn.1002-266X.2013.01.001
NT-3联合NSCs移植治疗乳鼠缺血缺氧性脑损伤的电生理评价
NT-3 jointed neural stem cell transplantation in the treatment of neonatal rats with hypoxic-ischemic brain injury electrophysiological evaluation
摘要
Abstract
Objective To observe neural electrophysiological manifestations of neonatarats with hypoxia ischemia brain damage(HIBD) in the treatment of neurotrophin-3 (NT-3) and neural stem cells (NSCs) transplantation. Methods Totally 10 7-day-old Wistar neonatal rats were randomly assigned to the control group, 50 7-day-old Wistar neonatal rats were selected to establish HIBD animal models, 37 cases were alive, and then randomly assigned to the NSCs transplantation group, NT-3 jointed NSCs transplantation group, brain injury group. NSCs, NSCs + NF3, PBS were transplanted in the injury side of the lateral ventricle respectively after 3 days. After transplanted for three weeks, seven weeks, the nerve electrophysiological was tested, then taking the brain tissue for HE staining. Results Neurophysiological testing; after 3 weeks for transplantation, compound action potential (CMAP) in rat hind limb quadriceps of rats with NT-3 combined NSCs transplantation or NSCs transplantation was significantly higher volatility, shorter incubation period compared with brain injury group( all P <0.05) ; after 7 weeks for transplantation, CMAP in rat hind limb quadriceps of rats with NT-3 combined NSCs transplantation was significantly higher volatility(P<0.05) ; HE staining; in brain injury group, damage lateral ventricle adjacent normal tissue structural damaged, there were a number of different sizes of the cysts, in NSCs transplantation group, periventricular normal organizational structure had been restored, and had rare cystic; in NT-3 jointed NSCs transplantation group, the ipsilateral periventricular tissue structure recovered more better. Conclusions NT-3 combined neural stem cell transplantation can make CAMP amplitude in hind limb quadriceps of HIBD rats increased, the incubation period shorted.关键词
脑损伤/干细胞/神经营养因子3/乳鼠Key words
brain injuries/ children/ stem cells/ neurotrophin 3/ rats分类
医药卫生引用本文复制引用
冯冠军,李凯,杨小朋,陈刚..NT-3联合NSCs移植治疗乳鼠缺血缺氧性脑损伤的电生理评价[J].山东医药,2013,53(1):1-4,4.基金项目
国家自然科学基金项目(30960393) （30960393）
无锡市卫生局科研计划项目(ML201211). （ML201211）
