山东医药2013，Vol.53Issue(4)：1-3,7,4.DOI:10.3969/j.issn.1002-266X.2013.04.01
甲型流感病毒H1N1亚型HA基因的克隆及序列分析
Cloning and bioinformatics analysis of influenza A virus subtype H1N1 hemagglutinin gene
摘要
Abstract
Objective To clone influenza A virus subtype H1N1 hemagglutinin (HA) gene, and do sequence analysis. Methods The total RNA was isolated by TRIzol reagent from influenza A virus subtype H1N1, the cDNA was gained by RT-PCR and then used as a template for PCR, amplified HA gene. Recombinant plasmid pET 28a( + )-HA was constructed. To analyze the nucleic acid sequence and properties of HA by bioinformatics analysis software. Results The recombinant plasmid pET 28a( + )-HA was constructed, sequence alignment result showed that the sequence shares 99% of sequence homology with the HA reported in GenBank. Conclusion The cloning and bioinformatics analysis of HA lay a foundation for the deeply study of its role in virus replication cycle and the in vitro screening of HA inhibitors.关键词
流感病毒/H1N1亚型/血凝素/克隆/序列分析Key words
influenza virus/ subtype HIM/ hemagglutinin/ cloning/ bioinformatics analysis分类
医药卫生引用本文复制引用
钟媚共,邱贤秀,向阳飞,吴崇超,王一飞..甲型流感病毒H1N1亚型HA基因的克隆及序列分析[J].山东医药,2013,53(4):1-3,7,4.基金项目
国家自然科学基金广东联合基金(U0632010) （U0632010）
广东高校皮肤黏膜释药制剂产学研结合示范(暨研究生创新培养)基地(cgzhzd0905). （暨研究生创新培养）
