实验动物与比较医学2012，Vol.32Issue(5)：421-425,5.DOI:10.3969/j.issn.1674-5817.2012.05.011
番茄红素对糖尿病大鼠肾组织血管紧张素转换酶2表达的影响
Effect of Lycopene on Angiotensin-converting Enzyme 2 Expression in Kidney Tissue of Rats with Diabetic Nephropathy
摘要
Abstract
Objective To observe the expression changes of angiotensin-converting enzyme 2 (ACE2) in kidney tissue of rats with diabetic nephropathy, and explore the renal protection mechanism of lycopene. Methods The rats were divided into 5 groups: nomal control group, diabetic nephropathy (DM) model group, and Lycopene treated groups with high-dose (20 mg·kg-1·d-1), middle-dose (10 mg·kg-1·d-1), and low-dose (5 mg·kg-1·d-1) respectively. After 8 weeks, the blood and urine index was measured. The expression level of ACE2 protein in kidney tissue was detected respectively by immunohis-tochemistry and western blotting. Results The concentrations of blood glucose, serum insulin, creatinine, urea nitrogen, Angll and urine protein in lycopene and diabetes groups were all significantly higher than those of normal control group (P<0.05); After treated by lycopene, serum creatinine, urea nitrogen, Angll and urine protein were greatly improved (P<0.05). The expression of ACE2 protein in diabetes group was greatly decreased than that of normal control group (P<0.01), while ACE2 protein levels in the high- and middle-dose groups treated with lycopene were obviously up-regulated (P<0.05), especially in high-dose lycopene group (P<0.01). Conclusion Lycopene can decrease urine protein, improve the renal function, and up-regulate the expression of ACE2 protein in diabetic nephropathy.关键词
番茄红素/糖尿病肾病/血管紧张素转化酶2Key words
Lycopene/ Diabetic nephropathy/ ACE2分类
生物科学引用本文复制引用
吴博,江海燕,王一龙,陈通克,朱加银,赵惠玲..番茄红素对糖尿病大鼠肾组织血管紧张素转换酶2表达的影响[J].实验动物与比较医学,2012,32(5):421-425,5.基金项目
浙江省实验动物科技计划项目(2009F80024) （2009F80024）
