兽类学报2012，Vol.32Issue(1)：48-57,10.
HS-SPME-GC/MS在大熊猫尿液化学成分检测中的应用
The application of HS-SPME-GC/MS in detecting chemical components in giant panda (Ailuropoda melanoleuca) urine
摘要
Abstract
Urine plays an important role in chemical communication between giant pandas (Ailuropoda melanoleuca). The detection of chemical compounds in giant panda urine is the key to identifying the compositions and assaying the function of each chemical compound. We used headspace-solid phase microextraction ( HS-SPME) and gas chromatography-mass spec-trometry (GC/MS) to pre-treat and analyze the composition of giant panda urine. We found 56 peaks and through searching the mass spectrometry library of the National Institute of Standards and Technology (NIST) , 38 of them were putatively I-dentified. In addition, we also modified the method of cleaning HS-SPME fiber. The results show that HS-SPME-GC/MS is an effective method to detect the volatile and semi-volatile compounds in giant panda urine. It is proved to be an important research tool for revealing the mechanism of chemical communication in the giant pandas.关键词
大熊猫/尿液/顶空固相微萃取/气相色谱-质谱联用/化学物质组成Key words
Chemical composition/ Gas Chromatography-Mass Spectrometry ( GC/MS) / Giant Panda/ Headspace-Solid Phase Microextraction ( HS-SPME) / Urine分类
化学化工引用本文复制引用
刘玉良,辛秋红,刘定震,蔡志刚,张志和,兰景超,魏荣平,李德生,侯蓉..HS-SPME-GC/MS在大熊猫尿液化学成分检测中的应用[J].兽类学报,2012,32(1):48-57,10.基金项目
成都市建委-大熊猫性外源激素研究项目 （）
