兽类学报2012，Vol.32Issue(4)：335-339,5.
梅花鹿S100A4基因RNAi重组慢病毒载体的构建
Construction of a lentiviral vector to target expression of S100A4 of sika deer (Cervus nippon)
摘要
Abstract
In this study, a number of sequences of small interfering RNA targeting S100A4 gene of sika deer ( Cervus nippon) were synthesized and screened, among which two were confirmed by BLAST search to be suitable for the purpose. 01-igo DNAs containing either sense or antisense strands were ligated into the lentiviral plasmids (pLVTHM) using T4 DNA ligase. Positive clones were identified based on the results of both PCR and sequencing. Each positive plasmid was co-transfected into 293T cells with the plasmids pCMV-dr 8. 91 and pMD2. G. Twenty-four hours after the co-transfection, green fluorescence of the 293 T cells could be observed under the inverted microscope. Therefore, we successfully constructed recombinant lentiviral system targeting sika deer S100A4 gene. This work would lay the foundation for revealing the regulatory mechanism of S100A4 underlying antler generation and regeneration.关键词
鹿茸/S100A4基因/293T/慢病毒载体/RNAiKey words
Antler/ 293 T/ Lentiviral vector/ RNAi/ S100A4 gene分类
生物科学引用本文复制引用
魏明立,褚文辉,赵海平,王大涛,孙红梅,李春义..梅花鹿S100A4基因RNAi重组慢病毒载体的构建[J].兽类学报,2012,32(4):335-339,5.基金项目
973前期研究专项(2011CB111515) （2011CB111515）
国家自然科学基金资助项目(31170950) （31170950）
吉林省自然科学基金资助项目(20101575) （20101575）
