色谱2013，Vol.31Issue(1)：83-87,5.DOI:10.3724/SP.J.1123.2012.08050
高效液相色谱-荧光检测法测定食品接触材料塑料制品中荧光增白剂
Determination of fluorescent whitening agents in plastic food contact materials by high performance liquid chromatography with fluorescence detector
摘要
Abstract
A method for the determination of fluorescent whitening agents in plastic food contact materials by high performance liquid chromatography (HPLC) with fluorescence detector was developed. The samples were extracted with trichloromethane by sonication for 30 min at 40 ℃. The HPLC method was performed on a column of Eclipse XDB-C18 (250 mm ×4. 6 mm, 5 μm) by gradient elution using 5 mmol/L ammonium acetate and acetonitrile as the mobile phases, and detected by the fluorescence detector at an excitation wavelength of 350 nm and an emission wavelength of 430 nm. The experimental results indicated that the four fluorescent whitening agents were separated well. The limits of detection (LOD) (S/N = 3) were 0. 3 , 0. 1, 0. 05 , 0. 14 mg/L, and the limits of quantification (LOQ) (S/N = 10) were 1.0, 0. 4, 0. 2, 0. 5 mg/L for 1, 4-bis (4-cyanostyryl) benzene ( C. I. 199 ), 1, 4-bis ( 2-benzoxazolyl) naphthalene (C.I. 367) , 4,4'-bis(2-methoxystyryl)biphenyl (C.I. 378) and 2,5-thiophenediylbis (5-tert-butyl-1,3-benzoxazole) (C.I. 184) , respectively. Good linearities with correlation coefficients (r2) not less than 0.991 were obtained. The proposed method is simple, accurate, sensitive and can meet the requirements of the routine determination of fluorescent whitening agents in entry-exit products.关键词
高效液相色谱/荧光检测/荧光增白剂/食品接触材料/塑料制品Key words
high performance liquid chromatography (HPLC)/ fluorescence detection/ fluorescent whitening agents (FWAs)/ food contact materials/ plastic分类
化学化工引用本文复制引用
焦艳娜,丁利,朱绍华,傅善良,龚强,李晖,王利兵..高效液相色谱-荧光检测法测定食品接触材料塑料制品中荧光增白剂[J].色谱,2013,31(1):83-87,5.基金项目
国家"十二五"科技支撑计划项目(2011BAK10B05,2012BAD29B05). （2011BAK10B05,2012BAD29B05）
