水生生物学报2012，Vol.36Issue(3)：433-440,8.DOI:10.3724/SP.J.1035.2012.00433
嗜麦芽寡养单胞菌脂多糖对斑点叉尾免疫保护作用
THE IMMUNOPROTECTION OF STENOTROPHOMONAS MALTOPHILIA LIPOPOLYSACCHARIDE IN CHANNEL CATFISH
摘要
Abstract
Stenotrophomonas maltophilia is a vital pathogenic bacteria of a high-lethal infectious diseases affecting channel catfish (Ictalurus punctatus) in recent years. In order to evaluate the immune protective effect of Stenotrophomonas maltophilia lipopolysaccharide (LPS) on channel catfish, four hundred healthy channel catfish were chosen and divided into I , II, III and IV groups randomly. Each group included two horizontal treatments which were treated with LPS, LPS plus polysaccharide of Ficus carica, whole cell inactivated vaccine and the saline source respectively by in-traperitoneal injection. The initial immunization was carried out at the beginning and so was the booster immunization at the 28 day. During the trial period, the blood leukocyte bactericidal activity, complement C3 levels, IgM levels and agglutinating antibody titers were determined at intervals of 7 days. The challenge with live Stenotrophomonas maltophilia was carried out at the 49th day. The results showed that the agglutinating antibody titers in the sera of channel catfish treated with LPS and LPS plus adjuvant of polysaccharide of Ficus carica. And the leukocyte bactericidal activity, complement C3 levels, IgM levels and the relative percentage survival of the fish treated with LPS, LPS plus polysaccharide of Ficus carica were both significant after the initial immunization. After the booster immunization, to the channel catfish treated with LPS and LPS plus adjuvant of polysaccharide of Ficus carica, the agglutinating antibody titers in the sera of those were 1 : 256 and 1 : 512, the leukocyte bactericidal activity of those were 0.565 and 0.511, the complement C3 levels of those were 0.194 and 0.180mg/mL, the IgM levels of those were 1.415 and 1.464mg/mL respectively, and the relative percentage survival of those were 70.0% and 65%, respectively. Conclusion: the above index of the fish treated with LPS and LPS plus polysaccharide of Ficus carica were significant (P<0.05) or most significant (P<0.01) than those treated with the whole cell inactivated vaccine and saline. The protective effect of LPS and LPS plus polysaccharide of Ficus carica on the channel catfish was better than the whole cell inactivated vaccine.关键词
嗜麦芽寡养单胞菌/斑点叉尾/脂多糖/无花果多糖/免疫Key words
Stenotrophomonas maltophilia/Channel catfish/LPS/Polysaccharide of Ficus carica/Immunization分类
农业科技引用本文复制引用
汪开毓,黄锦炉,肖丹,彭浪,黄艺丹..嗜麦芽寡养单胞菌脂多糖对斑点叉尾免疫保护作用[J].水生生物学报,2012,36(3):433-440,8.基金项目
教育部《长江学者和创新团队发展计划》创新团队项目(IRT0848) （IRT0848）
农业部动物检疫检测与防治项目(2130108) （2130108）
通威股份有限公司重点项目(2006-2009)资助 （2006-2009）
