水生生物学报2012，Vol.36Issue(3)：466-473,8.DOI:10.3724/SP.J.1035.2012.00466
饲料中糖/脂肪比例对瓦氏黄颡鱼生长、饲料利用、血糖水平和肝脏糖酵解酶活力的影响
EFFECTS OF DIETARY CARBOHYDRATE TO LIPID RATIO ON GROWTH,FEED UTILIZATION,PLASMA GLUCOSE AND GLYCOLYTIC ENZYME ACTIVITIES OF JUVENILE DARKBARBEL CATFISH,PELTEOBAGRUS VACHELLI
摘要
Abstract
An 8-week feeding trial was conducted to evaluate the effects of dietary carbohydrate-to-lipid (CHO : L) ratio on growth performance, feed utilization, plasma glucose and key glycolytic enzyme activities of juvenile darkbar-bel catfish (Pelteobagrus vachelli). Five isonitrogenous (crude protein 40%) and isoenergetic (19 MJ/g) diets were formulated to contain with graded levels of CHO : L ratios (0.75—6.53). Each diet was randomly assigned to triplicate tanks (50 L), and each tank was stocked with 40 fish with initial body weight of (4.20±0.02) g. The specific growth rate (SGR) increased first and then decreased with the increasing dietary CHO : L, and reached to the highest in fish fed diets with CHO : L of 3.55, which was significantly higher than those of fish fed the diets with 0.75 and 6.53 ratios (P<0.05). Feed efficiency (FE) and protein efficiency ratio (PER) showed no significant difference among treatments with CHO : L of 1.30—3.55, but FE and PER significantly decreased at CHO : L of 0.75 and 6.53 respectively (P<0.05). No significant difference was observed in the HSI and VSI of the darkbarbel catfish among dietary treatments (P>0.05). The crude protein of fish whole body increased first and then decreased with the increasing dietary CHO : L, and reached to the highest in fish fed diets with CHO : L of 3.55, which was significantly higher than that at CHO : L of 0.75 (P<0.05). The crude lipid of fish whole body decreased with the increasing dietary CHO : L significantly (P<0.05). There was no significant difference in the plasma glucose and total cholesterol among dietary treatments (P>0.05). Plasma triglycercide concentrations increased significantly with the increasing dietary CHO : L (P<0.05). Hepatic glucokinase (GK) activities increased first and then decreased with the increasing dietary CHO : L, and reached to the highest at the CHO : L of 3.55, which was significantly higher than those at CHO : L of 0.75 (P<0.05). However, no significant difference was observed in activities of hepatic hexokinase (HK), pyruvate kinase (PK) and 6-phosphofructo-l-kinase activity (PFK-1) among dietary treatments (P>0.05). Based on second-order polynomial regression analysis of SGR against dietary CHO : L ratios, a CHO : L ratio of 4.06 was proved to be optimal for the growth of juvenile darkbarbel catfish.关键词
瓦氏黄颡鱼/糖/脂比率/生长/糖酵解酶/摄食与营养Key words
Pelteobagrus vachelli/Carbohydrate to lipid ratio/Growth/Glycolytic enzymes/Feeding and nutrition分类
农业科技引用本文复制引用
张世亮,艾庆辉,徐玮,麦康森,刘付志国,张彦娇..饲料中糖/脂肪比例对瓦氏黄颡鱼生长、饲料利用、血糖水平和肝脏糖酵解酶活力的影响[J].水生生物学报,2012,36(3):466-473,8.基金项目
国家重点基础研究发展计划(973)项目(2009CB118702)资助 （973）
