生态学杂志2012,Vol.31Issue(12):3070-3074,5.
油菜素内酯对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响
Effects of brassinolide on the isozyme expressions of aerobic respiration enzymes in cucumber seedling roots under hypoxia stress
摘要
Abstract
A hydroponic experiment was conducted to study the effects of 24-epibrassinolide ( EBR) on the isozyme expressions of aerobic respiration enzymes in cucumber seedling roots un-der hypoxia stress. Hypoxia stress promoted the isozyme expressions of root isocitrate dehydrogen-ase (IDH) , succinate dehydrogenase ( SDH) , malate dehydrogenase ( MDH) , and malic en-zyme (ME) , and produced several new bands. Applying 10" mg·L-1 of EBR under hypoxia stress increased the isozyme expressions of IDH and MDH on day 6 and day 9 by 52. 8% and 13. 6% , and 39. 1% and 11.3% , respectively and the isozyme expressions of ME on day 3 by 11. 6% , but decreased the isozyme expressions of SDH on day 6 and day 9 by 42. 9% and 36. 1 % , respectively, as compared with those under hypoxia stress alone. These results indicated that applying EBR under hypoxia stress could regulate the isozyme expressions of IDH, SDH, MDH, and ME in cucumber seedling roots, and thereby, alleviate the damage of hypoxia stress on the seedling roots.关键词
黄瓜/低氧胁迫/24-表油菜素内酯/有氧呼吸/同工酶Key words
cucumber/hypoxia stress/24-epibrassinolide/aerobic respiration/isozyme分类
农业科技引用本文复制引用
陆晓民,孙锦,郭世荣,何立中,王丽萍,阳燕娟..油菜素内酯对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响[J].生态学杂志,2012,31(12):3070-3074,5.基金项目
国家自然科学基金项目(30871736)、国家重点基础研究发展计划项目(2009CB119000)、现代农业产业技术体系建设专项(CARS-25-C-03)、江苏省农业三项工程项目(SX(2011)289)和江苏高校优势学科建设工程项目资助. (30871736)