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油菜素内酯对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响

陆晓民 孙锦 郭世荣 何立中 王丽萍 阳燕娟

生态学杂志2012,Vol.31Issue(12):3070-3074,5.
生态学杂志2012,Vol.31Issue(12):3070-3074,5.

油菜素内酯对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响

Effects of brassinolide on the isozyme expressions of aerobic respiration enzymes in cucumber seedling roots under hypoxia stress

陆晓民 1孙锦 2郭世荣 1何立中 1王丽萍 1阳燕娟1

作者信息

  • 1. 南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,南京210095
  • 2. 安徽科技学院,安徽凤阳233100
  • 折叠

摘要

Abstract

A hydroponic experiment was conducted to study the effects of 24-epibrassinolide ( EBR) on the isozyme expressions of aerobic respiration enzymes in cucumber seedling roots un-der hypoxia stress. Hypoxia stress promoted the isozyme expressions of root isocitrate dehydrogen-ase (IDH) , succinate dehydrogenase ( SDH) , malate dehydrogenase ( MDH) , and malic en-zyme (ME) , and produced several new bands. Applying 10" mg·L-1 of EBR under hypoxia stress increased the isozyme expressions of IDH and MDH on day 6 and day 9 by 52. 8% and 13. 6% , and 39. 1% and 11.3% , respectively and the isozyme expressions of ME on day 3 by 11. 6% , but decreased the isozyme expressions of SDH on day 6 and day 9 by 42. 9% and 36. 1 % , respectively, as compared with those under hypoxia stress alone. These results indicated that applying EBR under hypoxia stress could regulate the isozyme expressions of IDH, SDH, MDH, and ME in cucumber seedling roots, and thereby, alleviate the damage of hypoxia stress on the seedling roots.

关键词

黄瓜/低氧胁迫/24-表油菜素内酯/有氧呼吸/同工酶

Key words

cucumber/hypoxia stress/24-epibrassinolide/aerobic respiration/isozyme

分类

农业科技

引用本文复制引用

陆晓民,孙锦,郭世荣,何立中,王丽萍,阳燕娟..油菜素内酯对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响[J].生态学杂志,2012,31(12):3070-3074,5.

基金项目

国家自然科学基金项目(30871736)、国家重点基础研究发展计划项目(2009CB119000)、现代农业产业技术体系建设专项(CARS-25-C-03)、江苏省农业三项工程项目(SX(2011)289)和江苏高校优势学科建设工程项目资助. (30871736)

生态学杂志

OA北大核心CSCDCSTPCD

1000-4890

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