生态学杂志2012，Vol.31Issue(12)：3179-3186,8.
假臭草从枝病植原体鉴定与多样性分析
Identification and diversity analysis of phytoplasma associated with Praxelis clematidea witch' s broom
摘要
Abstract
To study the diversity and classification of phytoplasma associated with Praxelis clema-tidea witch' s broom is of significance for the bio-control of the pathogen. In this paper, the P. clematidea plants with the presence of the phytoplasma were collected from eight counties in Hainan Province of South China. The samples were tested by PCR amplification using phytoplas-ma-specific 16S rDNA primers. Furthermore, the diversity of the phytoplasma associated with P. clematidea witch ' s broom was investigated with restricted fragment length polymorphisms ( RFLP) by using the restriction enzymes Afa Ⅰ, Alu Ⅰ, EcoR Ⅰ, Hae Ⅲ , Hpa Ⅱ , Hha Ⅰ, Hinf Ⅰ, Kpn Ⅰ, Sau3A Ⅰ, Taq Ⅰ, and Xsp Ⅰ. The PCR products were cloned and sequenced, and the se-quences were compared with the members of 16SrⅡ group of phytoplasma. The results showed that the causal agent of P. clematidea witch' s broom was the phytoplasma. The patterns of RFLP analysis of 16S rDNA sequence primed from the phytoplasma collected from the eight counties were basically the same, and the similarity between the 16S rDNA and that of other known phyto-plasmas ranged from 0.26 to 0.97. There was a high similarity ( >99.4% ) within the sequences from the eight counties, and the highest similarity (99. 1% ) with the phytoplasma from peanut witch' s broom. It was suggested that the phytoplasma of P. clematidea witch' s broom could be categorized as a branch of 16S rRNA Ⅱ -A group.关键词
假臭草/丛枝病/植原体/16S rDNA/RFLP分析Key words
Praxelis clematidea/witch's broom/phytoplasma/16S rDNA/restricted fragment length polymorphism (RFLP) analysis分类
农业科技引用本文复制引用
李光明,袁坤,杨礼富,陈秋波,王真辉..假臭草从枝病植原体鉴定与多样性分析[J].生态学杂志,2012,31(12):3179-3186,8.基金项目
国家自然科学基金项目(30960088)资助. （30960088）
