生态学杂志2012，Vol.31Issue(12)：3187-3192,6.
酿酒酵母和克鲁维酵母发酵菊芋生产燃料乙醇
Ethanol production from Jerusalem artichoke through the fermentation with Saccharomyces cerevisiae and Kluyveromyces
摘要
Abstract
In order to obtain the optimal combination of bacterial strains for the fermentation pro-duction of ethanol from Jerusalem artichoke ( Helianthus tuberosus) , the recombinant bacterial strain R32 was selected at first to optimize its inulinase producing conditions. The highest inuli-nase activity of the R32 was 298. 8 U·mL-1 , and the best culture medium formulae were 1% yeast powder (w/v) , 2% peptone (w/v) , and 0.5% glycerol (v/v) for YPG culture medium, and 1% yeast powder (w/v) , 2% peptone (w/v) , and 1% methanol (v/v) for YPM culture medium. The medium pH was the natural initial pH. Then, the Saccharomyces cerevisiae (S. c) and Kluyveromyces (Klu) were selected to compare their ethanol producing capability when fer-mented separately or in admixture under the conditions of adding R32 crude inulinase broth or not. The results showed that the ethanol production reached the maximum of 11. 37% after S. c and Klu being one-step fermented in admixture for 84 h without adding R32 crude inulinase broth, and reached the maximum of 11.43% after 5. c and Klu being two-step fermented in ad-mixture for 72 h with the addition of R32 crude inulinase broth. The maximum ethanol yields un-der the two-step fermentation of S. c and Klu were almost the same. Because of the lower cost and more simplicity of operation, one-step fermentation was more appropriate for the industrial produc-tion of ethanol, though it took a longer time. After optimization, the production of ethanoJ from Je- rusalem artichoke by 5. c and Klu mixed in one-step fermentation was up to 11.82% under the con-ditions of 225 g·L-1 of inulin, 40 g·L-1 of urea, 15% of inoculation rate, and pH 5.关键词
菊芋/乙醇/克鲁维酵母/酿酒酵母/发酵Key words
Jerusalem artichoke/ethanol/Kluyveromyces/Saccharomyces cerevisiae/fermenta-tion分类
化学化工引用本文复制引用
黄玉玲,隆小华,刘兆普,王琳,王博..酿酒酵母和克鲁维酵母发酵菊芋生产燃料乙醇[J].生态学杂志,2012,31(12):3187-3192,6.基金项目
公益性行业(农业)科研专项经费项目(200903001-05)、国家科技支撑项目(2011BAD13B09)、中央高校基本业务费项目(Y0201100249)、教育部博士点基金项目(20100097120016)和江苏省科技支撑项目(BE2010305,BE2011368)资助. （农业）
