生态学杂志2013，Vol.32Issue(2)：368-374,7.
不同水生植物残体分解过程中真菌群落结构
Fungal community structure during the decomposition of various aquatic plant litters
摘要
Abstract
Aquatic plants are the main primary producers in shallow lake, and their decomposi-tion and mineralization have significant effects on the nutrient cycle in lake ecosystems. Fungi are one of the major decomposers in natural environments, but little is known about the composition and functions of fungal community during the decomposition of various aquatic plant litters. In this paper, various types of aquatic plant residues were separately added into undisturbed lake sediments and their upper water to study the characteristics of fungal community structure during the decomposition of these residues, based on the selection of suitable primers that can specifical-ly amplify fungal 18S rRNA gene and on the construction of clone libraries and sequencing. The results showed that the primer pairs nu-SSU-0817/nu-SSU-1536 and nu-SSU-0817/nu-SSU-1196 were suitable to amplify the fungal 18S rRNA gene. Basidiomycota was the most common fungal taxa in water column, and Chytridiomycota was the most common one in surface sediments. Sta-tistical analysis showed that there existed obvious differences in the fungal community structure in water column and surface sediments. This study would give further insight into the functions and niche differentiation of fungi during the decomposition of aquatic plant litters.关键词
真菌/18S rRNA基因/巢式PCR/克隆建库Key words
fungi/18S rRNA gene/nested PCR/clone library分类
资源环境引用本文复制引用
郑九文,邢鹏,余多慰,郭远..不同水生植物残体分解过程中真菌群落结构[J].生态学杂志,2013,32(2):368-374,7.基金项目
国家自然科学基金项目(31000241)资助. （31000241）
