生物加工过程2012，Vol.10Issue(6)：1-6,6.DOI:10.3969/j.issn.1672-3678.2012.06.001
树干毕赤酵母和酿酒酵母混合糖发酵产乙醇
Co-fermentation of mixture of glucose and xylose to ethanol by Pichia stipitis and Saccharomyces cerevisiae
摘要
Abstract
Acid steam explosion pre-hydrolysis of corn stalk and pure sugar analog fluid fermented by Pichia stipites and Saccharomyces cerevisiae. The effects of initial pH of pre-hydrolysis of corn stalk, the effect of type and concentration of nitrogen and three fermentation patterns on fermentation of P. stipites were studied. The result showed that the initial pH range of pre-hydrolysis of corn stalk for fermentation was pH 6. 0 to pH 7. 0. 1.0g/L (NH4)2SO4 was used as the nitrogen source, in 40 g/L glucose and 25 g/L xylose fermentation medium for 24 h,and sugar utilization was 99.47% , ethanol concentration was 24. 72 g/L, which was superior to urea and peptone as nitrogen source. Fermentation performances of free P. stipitis cell and immobilized S. cerevisiae were the best, sugar utilization and ethanol yield were 99.43%, and 96.39%. Immobilized yeast could achieve high-density fermentation, thus improving equipment utilization and having a better prospect on multi-stage continuous fermentation in the bioreactor.关键词
树干毕赤酵母/酿酒酵母/固定化细胞/乙醇Key words
Pichia stipitis /Saccharomyces cerevisiae/immobilized cells/ethanol分类
化学化工引用本文复制引用
宋向阳,李全,欧阳嘉,勇强..树干毕赤酵母和酿酒酵母混合糖发酵产乙醇[J].生物加工过程,2012,10(6):1-6,6.基金项目
国家自然科学基金资助项目(31070513) （31070513）
江苏省高校优势资源学科建设工程资助项目(PAPD) （PAPD）
江苏高校科技创新团队资助项目 （）
