生物加工过程2012，Vol.10Issue(6)：7-11,5.DOI:10.3969/j.issn.1672-3678.2012.06.002
耐有机溶剂蛋白酶产生菌Bacillus cereus WQ9-2发酵条件及酶学性质
Optimum fermentation conditions and enzymatic properties of organic solvent tolerant protease from Bacillus cereus WQ9-2
摘要
Abstract
In order to raise protease production,culture conditions for Bacillus cereus WQ9-2 with the ability to produce organic solvent tolerant protease were optimized by central composite design method. The optimized fermentation conditions were as follows; yeast powder 8 g/L, glucose 17 g/L, KH2PO4 0. 5 g/L, MgSO40. 3 g/L, CaCl2 0.5 g/L, NaCl 1. 0 g/L, and pH 7. 5. The cultivation conditions were 25 °C , and 180 r/min. Under the optimal conditions for 48 h,the maximum protease production was 3 921 U/mL. The protease activity was activated in the presence of Ca +, and inhibited in the presence of EDTA and 1 ,10-phenanthroline. The results showed that it might be metalloprotease.关键词
Bacillus cereus/耐有机溶剂蛋白酶/优化/酶学性质Key words
Bacillus cereus/organic solvent tolerant protease/optimization/enzymatic property分类
生物科学引用本文复制引用
许家兴,李振东,承龙飞,何冰芳..耐有机溶剂蛋白酶产生菌Bacillus cereus WQ9-2发酵条件及酶学性质[J].生物加工过程,2012,10(6):7-11,5.基金项目
国家高技术发展计划(863计划)资助项目(2006AA02Z202) （863计划）
江苏省普通高校研究生科研创新计划资助项目(CX10B_176Z) （CX10B_176Z）
