生物加工过程2013，Vol.11Issue(1)：17-22,6.DOI:10.3969/j.issn.1672-3678.2013.01.004
羰基还原酶不对称还原(R)-6-氰基-5-羟基-3-羰基己酸叔丁酯
Asymmetric reduction of t-butyl 6-cyano-(5R)-hydroxy-3-carbonylhexanoate by carbonyl reductase
摘要
Abstract
t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate,a key chiral building block for atorvastatin,was synthesized through asymmetric reduction of ( R )-6-cyano-5-hydroxy-3-carbonylhexanoate catalyzed by carbonyl reductase coupled with glucose dehydrogenase. The optimized biocatalytic conditions were obtained; (R)-6-cyano-5-hydroxy-3-carbonylhexanoate 147.0 g/L,glucose 128.2 g/L,cell loading 15.0 g/L,the pH 6. 5 , and 30 ℃. Under these conditions, (R)-6-cyano-5-hydroxy-3-carbonylhexanoate was completly converted for 6 h. Without exogenous addition of NADP(H) and with t-butyl 6-cyano-(3R, 5R) -dihydroxyhexanoate the d. e. value was over 99.5% .关键词
不对称生物还原/阿托伐他汀/6-氰基-(3R,5R)-二羟基己酸叔丁酯/辅酶再生Key words
asymmetric bioreduction/atorvastatin/t-butyl 6-cyano-( 3R, 5R )-dihydroxyhexanoate/coenzyme regeneration分类
化学化工引用本文复制引用
曹政,王亚军,肖黎,柳志强,郑裕国..羰基还原酶不对称还原(R)-6-氰基-5-羟基-3-羰基己酸叔丁酯[J].生物加工过程,2013,11(1):17-22,6.基金项目
国家重点基础研究发展计划(973计划)资助(2011CB710800) （973计划）
