生物加工过程2013，Vol.11Issue(1)：35-40,6.DOI:10.3969/j.issn.1672-3678.2013.01.007
一株高产木聚糖酶真菌的筛选及酶学性质分析
Screening of xylanase-producing fungus and analysis of enzymatic properties
摘要
Abstract
Hundreds of various soil samples were collected. Firstly,564 strains with halo-forming zones were isolated. Then, the xylanase activities of 202 strains were further verified by a 96-deep-well microplate screening assay. The activities from 96-deep-well microplate and halo-forming plate showed the same trend. The best strain ECU2023 with the highest xylanase production was identified as Aspergillus awamori by internal transcnbed spacer (ITS) sequencing. The culture conditions were optimized as follows:30 ~ 40 g/L corn cob powder, pH 6. 0, cultivation for 4 d. Furthermore, the main xylanase component was crudely was purified by ammonium sulfate precipitation and Superdex G-75 chromatograph. The xylanase activity was optimally performed at 50 ℃ and pH 6.0.关键词
木聚糖酶/真菌/菌株筛选/96孔深孔板/玉米芯粉Key words
xylanase/fungi/strain screening/96-deep-well plate/corn cob分类
农业科技引用本文复制引用
孙婕,郁惠蕾,李春秀,寇振福,许建和..一株高产木聚糖酶真菌的筛选及酶学性质分析[J].生物加工过程,2013,11(1):35-40,6.基金项目
国家重点基础研究发展计划(973计划)资助(2011CB710803) （973计划）
