生物加工过程2013，Vol.11Issue(1)：104-112,9.DOI:10.3969/j.issn.1672-3678.2013.01.017
工业属性普鲁兰酶的开发及其催化性能改善的研究进展
Research advances on discovery and development of pullulanase with industrial properties
摘要
Abstract
Pullulanase is a kind of direct endo-acting de-branching enzyme that specifically catalyzes the hydrolysis of α-1,6-glucosidic linkages of unmodified substrate to give linear oligosaceharides, and mainly used in food industry. However, so far the available pullulanase is yet limited to meet the industrial requirements. Resent developments in advanced biotechnology have provided some approaches for highly efficient screening, expression and engineering of pulluanase with industrial properties. Otherwise, the substrate modification and the immobilization are adopted to improve the catalytic function of the enzyme at some level. This paper mainly summarizes the research advances in discovery, expression and engineering of pullulanase for the development of its application potential.关键词
普鲁兰酶/产酶菌种/异源表达/结构分析/热稳定性Key words
pullulanase/enzyme source organism/expression/structure analysis/thermostability分类
生物科学引用本文复制引用
聂尧,严伟,徐岩..工业属性普鲁兰酶的开发及其催化性能改善的研究进展[J].生物加工过程,2013,11(1):104-112,9.基金项目
国家重点基础研究发展计划(973计划)资助(2011CB710800) （973计划）
国家高技术研究发展计划(863计划)资助(2012AA022207) （863计划）
高等学校学科创新引智计划(111计划)资助(111-2-06) （111计划）
江苏高校优势学科建设工程资助 （）
