山西医科大学学报2012，Vol.43Issue(11)：805-808,4.DOI:10.3969/J.ISSN.1007-6611.2012.11.001
维生素C对人胚肺成纤维细胞增殖抑制及凋亡的影响
Effect of vitamin C on inhibition of proliferation and apoptosis of human embryo fibroblasts
翟鹏勇 1吴国瑾 1梁淑霞 1李雪琴 1王燕1
作者信息
- 1. 山西晋城晋煤集团总医院呼吸内科, 晋城048006
- 折叠
摘要
Abstract
Objective To explore the proliferation and apoptosis of human embryo fibroblast-Ⅰ ( HFL- Ⅰ ) cell lines after cultured with vitamin C. Methods HFL- Ⅰ cell lines were cultured with 0,40,400,4 000 μg/ml vitamin C,respectively. The cell viability was measured by CCK-8 assay. Flow cytometry was used to analyze the cell cycle. Collagen expression was measured by Sirius red staining. The expression of Caspase-3 mRNA was detected by reverse transeription-polymerase chain reaction(RT-PCR). Results Vitamin C inhibited the growth of HFL- I cell lines in a dose-dependent manner(P ＜ 0.05) , and reduced the formation of collagen(P ＜ 0.05). Flow cytometry results showed that the cells were delayed in the G1 phase. Vitamin C significantly up-regulated the mRNA of Caspase-3 after cultured with vitamin C for 12 h. Conclusion Vitamin C can inhibit the proliferation of HFL- Ⅰ , reduce the formation of collagen, block HFL-Ⅰ cells in G1 phase,and induce the apoptosis of HFL-Ⅰ cells by up-regulating the expression of Caspase-3 mRNA.关键词
维生素C/人胚肺成纤维细胞/胶原/凋亡Key words
vitamin C/ human embryo fibroblast/ collagen/ apoptosis分类
医药卫生引用本文复制引用
翟鹏勇,吴国瑾,梁淑霞,李雪琴,王燕..维生素C对人胚肺成纤维细胞增殖抑制及凋亡的影响[J].山西医科大学学报,2012,43(11):805-808,4.
