山西医科大学学报2013，Vol.44Issue(1)：5-8,4.DOI:10.3969/J.ISSN.1007-6611.2013.01.002
BALB/c小鼠神经母细胞瘤移植模型的建立及相关影响因素的研究
Establishment and related influential factors analysis of neuroblastoma xenograft model in BALB/c mice
张锐虎 1荣曼 1刘田福1
作者信息
- 1. 山西医科大学实验动物中心,太原,030001
- 折叠
摘要
Abstract
Objective To establish the mouse neuroblastoma xenograft model in BALB/c mice, and to explore the influential factors for the successful rate of modeling. Methods BALB/c mice were randomly divided into three groups: abdominal cavity group, nape group and oxter group. Neuro-2a( N2a )cells were collected and condensed as 1 × 10 /ml during the logarithmic growth phase. BALB/c mice were injected subcutaneously 0. 2 ml N2a cells through abdominal cavity, nape and oxter,respectively. The mice were injected for the first cycle at days 1-7 after born. Based on the examination of appearance and touch,the mice without neuroblastoma were injected a-gain for the second cycle( 8-14 d ), and even for the third cycles( 15-21 d )if necessary in order to have successful inoculation. The latent period and rate of tumor bearing were recorded, and the growth curves of nomal mice and the ones bearing cancer. Results The survival rate of mice was 94. 34%( 50/53 )and the tumour-bearing rate was 42% . Of all the tumor-bearing mice,tumour formation rate was 46. 2% via abdominal cavity, 38. 1% via nape and 33. 3% via oxter. The tumor-bearing rate of female mice was higher than that of male ones( 57. 89% vs 32. 26% ,P ＜ 0. 05 ). The tumor generation rate decreased with the aging, and the tumour formation rate was 48. 2% for 1 -7 d old mice,35. 7% for 8 - 14 d old mice and 20% for 15 -21 d old mice. The shortest latency was 5 d( 4. 76% ),and the longest was 20 d( 9. 53% ), while the most was 11 - 15 d( 61. 9% ). Finally the mice bearing cancer died of excessively bigger neu-roblastoma and skeletization. Conclusion Mouse neuroblastoma xenograft model is successfully established in the immunocompetent BALB/c mice,which paves the way for the further research on pathogenesis and therapy of neuroblastoma.关键词
BALB/c小鼠/N2a细胞/动物模型Key words
BALB/c mice/ N2 a cells/ animal model分类
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张锐虎,荣曼,刘田福..BALB/c小鼠神经母细胞瘤移植模型的建立及相关影响因素的研究[J].山西医科大学学报,2013,44(1):5-8,4.
