现代检验医学杂志2012，Vol.27Issue(6)：25-29,5.DOI:10.3969/j.issn.1671-7414.2012.06.009
H2S对急性肺损伤大鼠PMN功能的影响机制
Mechanism of the Effect of H2S on PMN Function in Acute Lung injury Rats
代庆春 1李娜 2苗晓云 1韩玉 1周晓红 3张晓卫1
作者信息
- 1. 河北省沧州市中心医院重症医学科,河北沧州,061001
- 2. 河北省沧州市人民医院医务科,河北沧州,061000
- 3. 河北医科大学基础课部病理教研室,石家庄,050017
- 折叠
摘要
Abstract
Objective To observe the effect of hydrogen sulfide (H2S) on polymorphonuclear (PMN) function in lipopolysac-charide (LPS)-induced acute lung injury (ALI) rats. Methods 112 Sprague-Dawley rats were randomly divided into four groups:sham-operated group,model group(LPS were instilled intratracheally to induce ALI), LPS+NaHS (H2S donor) group,and LPS+PPG (propargylglycin, CSE inhibitor) group. Then according to the dosage time divided into two subgroups (group 4h and group 8h). All the rats were observed at 4 h (14 rats) or 8 h (14 rats)after agent administration. The blood PMN CDllb expression was detected by Flow cytometry. Seven rats of each subgroups,Lung tissue myeloperoxidase (MPO) activity was used to detect by Neighbors even fennel amine method. ICAM-1 protein content in lung tissues was calculated to measure by double antibody sandwich elisa method. Lung histomorphological changes were observed under microscope and index of quantitative assessment (IQA) was calculated to measure the lung injury severity. Seven rats of each subgroups were received bronchoalveolar lavage. PMN number in Bronchoalveolar lavage fluid (BALF) was counted under light microscope. Results Compared with sham-operated group,severe injuries of lung tissues and a raised IQA,a raised PMN number in BALF,expression of ICAM-1 protein and expression of MPO protein were observed in rats treated with LPS. Pre-administration of NaHS not only alleviated the injuries induced by LPS but also decreased IQA,PMN number in BALF,expression of ICAM-1 protein and expression of MPO protein. Pre-administration of PPG exacerbated the injuries induced by LPS. Also,increased IQA,PMN number in BALF,expression of ICAM-1 protein and expression of MPO protein were observed. (Pall＜0. 01 or Pall＜0. 05, F value = 40. 133 or 45. 263; F value = 32. 760 or 54. 722; F value = 22. 256 or 35. 168; F value = 85. 223 or 98. 187）. Correlation analysis results showed that there was positive correlation between MPO activity in lung tissues and PMN number in BALF of rats ( r=0. 789, P<0. 01). Conclusion H2S can inhibit PMN function of ALI rats by reducing ICAM-1 ,CDllb expression and MPO activity.关键词
硫化氢/急性肺损伤/内毒素/脂多糖/中性粒细胞/黏附因子-1/CD11b/髓过氧化物酶Key words
hydrogen sulfide/ acute lung injury/ endotoxin/ lipopolysaccharide/ neutrophils/ adhesion molecule-1/ expression of CD11 b/ myeloperoxidase分类
医药卫生引用本文复制引用
代庆春,李娜,苗晓云,韩玉,周晓红,张晓卫..H2S对急性肺损伤大鼠PMN功能的影响机制[J].现代检验医学杂志,2012,27(6):25-29,5.
