现代检验医学杂志2012，Vol.27Issue(6)：41-43,46,4.DOI:10.3969/j.issn.1671-7414.2012.06.013
广州地区临床分离多重耐药肠杆菌科细菌的耐药基因检测
Detecting Resistant Gene of Multidug-resistaant Enterobacteriaceae from Clinical in Guangzhou
摘要
Abstract
Objective To detect resistant gene in clinical application Enterobacteriaceae strains and exploreits characteristics. Methods Biomerieux's VITEK-2 system was used to identify Enterobacteriaceae and its drug susceptibility. The double disk synergy test for detecting metallo-β-Lactamase of Enterobacteriaceae. The Metallo-β-Lactamases gene, qacE△l-sull gene,qnr genes and class 1,2,3 integrons carried on Enterobacteriaceae strains were detected by polymerase chain reaction (PCR)method. Results The positive rate of integron 1 of 109 from 115 strains was 94.8%. Class 2,3 integrons were not found. 107 of 115 strainswere qacE△l-sull gene positive. The positive rate of qnrA, qnrB, qnrS were 53. 9% ,12. 2% and 5.2% respectively. Nine strains were MBL-positive using EDTA double disk synergy test. This 9 strains was only susceptible to amikacin and polymyxin B. The IMP-4 and IMP-1 metallo-β-Lactamases were positive, meanwhile qnrA, qnrS, qacE △l-sull and intll were positive. Conclusion Especially ,producesd metal β-lactamase enterobacteriaceae bacteria appear to be highly multiple drug resistance and carry a variety of drug resistance gene. Integrons were important molecular mechanism in the development of multidrug resistance.关键词
碳青霉烯酶/Ⅰ类整合子/肠杆菌科细菌/耐药基因Key words
carbapenemase/class 1 integrons/ enterobacteriaceae/resistant gene分类
医药卫生引用本文复制引用
黄乐升,陈惠玲,李焕庭,李莉,叶惠芬..广州地区临床分离多重耐药肠杆菌科细菌的耐药基因检测[J].现代检验医学杂志,2012,27(6):41-43,46,4.基金项目
广东省科技厅研究基金资助项目(2011B031800093). （2011B031800093）
