现代检验医学杂志2012，Vol.27Issue(6)：76-78,3.DOI:10.3969/j.issn.1671-7414.2012.06.024
风疹病毒感染三种实验诊断方法应用分析
Evaluation of Laboratory Diagnosis Methods in Rubella Virus Infection
吴瑞 1杜全丽 1黄银娟 1李一为 1马超锋1
作者信息
- 1. 西安市疾病预防控制中心,西安,710054
- 折叠
摘要
Abstract
Objective To compare the three laboratory diagnosis methods in rubella virus infection. Methods 47 throat swabs and sera collected simultaneously in rubella outbreaks and sporadic cases in Xi'an from 2009 to 2011 were detected respectively by ELISA for rubella IgM,RT-PCR for viral RNA and viral isolation with colorimetric immunoassay( VICI) for virus. Results The positive rate of Elisa,RT-PCR and VICI respectively was 78. 7%, 76. 6% and 36. 2%, ELISA and RT-PCR had no significant difference but the VICI showed significantly different. The IgM positive rate reached 100% after 4 days of eruption,and the RT-PCR positive rate was 100% in 3 days of eruption,and the VICI positive rate was 68. 42% in 3 days of eruption. Conclusion For RUV infection laboratory diagnosis,ELISA and RT-PCR showed almost same results but ELISA is easy operating and could provide reliable results for the later period of eruption,and RT-PCR is specific and sensitive and is suitable for early diagnosis,and VICI is time consuming,overlaborate and with low positive rate.关键词
风疹病毒/酶联免疫吸附试验(ELISA)/逆转录聚合酶链反应(RT-PCR)/病毒分离免疫比色法(VICI)Key words
rubella virus/ ELISA/ RT-PCR/ viral isolation with colorimetric immunoassay分类
医药卫生引用本文复制引用
吴瑞,杜全丽,黄银娟,李一为,马超锋..风疹病毒感染三种实验诊断方法应用分析[J].现代检验医学杂志,2012,27(6):76-78,3.
