现代检验医学杂志2012，Vol.27Issue(6)：102-104,3.DOI:10.3969/j.issn.1671-7414.2012.06.032
拭样增菌对实时荧光PCR法检测孕妇B族链球菌感染的影响
Effect of Swabs Enrichment on the Detection of Group B-Streptococcal Infection in Gravid Women by Real-time Fluorescent PCR
边佳明 1丁媛媛 1杨凡 1张梅 1许景峰1
作者信息
- 1. 北京军区总医院药理科临床基因扩增检验室,北京,100700
- 折叠
摘要
Abstract
Objective To compare the effect of swabs enrichment on the detection of group B-streptococcal (GBS) infection by real-time fluorescent PCR in birth canal of gravid women. Methods 1 000 vagina-rectum swabs at 35 ~ 37 week's gestation were collected and evenly divided into enrichment group and non-enrichment group. Swabs of enrichment group were enriched in sterile broth overnight at room temperature followed by storage at 4℃ ready for detection, while swabs of non-enrichment group were stored at 4℃ ready for detection. All PCR detections must be completed within 48h to statistic GBS i-solating rate. Results Chisquare test showed that enrichment before DNA extraction remarkably affected isolating rate of GBS by real-time fluorescent PCR,and enrichment group had a higher isolating rate (1. 7 times of control,P=0. 006) than non-enrichment group. Conclusion The proper enrichment for clinical specimens is very feasible in clinical gene amplification test lab,by which false-negative was greatiyavoided,improving the value of GBS screen before delivery.关键词
实时荧光PCR/B族链球菌/围产期Key words
real-time PCR/group B-streptococcus/perinatal分类
医药卫生引用本文复制引用
边佳明,丁媛媛,杨凡,张梅,许景峰..拭样增菌对实时荧光PCR法检测孕妇B族链球菌感染的影响[J].现代检验医学杂志,2012,27(6):102-104,3.
