现代检验医学杂志2013，Vol.28Issue(1)：13-16,20,5.DOI:10.3969/j.issn.1671-7414.2013.01.005
湖北地区慢性丙型肝炎患者HCV RNA基因测序分型及疗效评估
Gene Sequence-Based Genotyping and Therapy Evaluation of Hepatitis C Virus in 168 Patients with Chronic Hepatitis C Infection in Hubei Province
摘要
Abstract
Objective To detect the genotype of hepatitis C virus in chronic hepatitis C infection patitents in Hubei province using gene sequence method,observe the distributive characteristic of HCV genotype in these area,and to explore the rela tionship among genotype,baseline HCV RNA,and antiviral effectsMethods The venous whole blood was drawn from chro nic hepatitis C patients in Renming Hospital of Wuhan University and RNA was extracted, then mRNA was converted to cDNA by reverse transcriptase. PCR products were purified by kit,then were sequenced by ABI3130 gene sequence analyzer. The results of gene sequence were compared with NCBI genebank data to obtain the HCV genotypes in patients. Some pa tients were selected for the evaluation of antiviral therapy results, baseline HCV RNA level and HCV genotype. Results The genotypes detected in the study were lb,2a,6b,lb/2a,lb/2k,and 6d/6k respectively. 149 (89%) cases were genotype lb,8(5%) cases were genotype 2a,5 (3%) cases were genotype lb/2a,3(2%) cases were genotype 6b,2 cases were geno type lb/2k and 1 case was genotype 6d/6k. The percentage of Genotype lb patients was significantly higher than that of oth er genotypes (X2 =754. 3, P<0. 01). RVR of genotype non lb patients was significantly higher than genotype lb patients (X2 =4. 67,P <0. 05). Conclusion Genotype lb was the major type of HCV in chronic hepatitis C patients in Hubei Prov ince. Better rapid therapy effects could be found in Peginterferon and ribavirin treated genotype non lb patients than that of lb patients.关键词
丙型肝炎病毒/基因测序/基因型Key words
hepatitis C virus/gene sequence/genotyping分类
医药卫生引用本文复制引用
包安裕,李艳,童永清,郑红云,吴薇..湖北地区慢性丙型肝炎患者HCV RNA基因测序分型及疗效评估[J].现代检验医学杂志,2013,28(1):13-16,20,5.基金项目
本课题获得国家临床重点专科建设项目的资助[财社(2010)305号]. （2010）
