现代检验医学杂志2013，Vol.28Issue(1)：21-23,3.DOI:10.3969/j.issn.1671-7414.2013.01.007
淋巴细胞亚群在肾移植术后巨细胞病毒感染患者中的表达及意义
Expression and Significance of Lymphocyte Subsets in Renal Transplantation Patients with Cytomegalovirus Infection
高钰 1肖漓 1石炳毅 1冯凯 1马锡慧 1孔祥瑞1
作者信息
- 1. 中国人民解放军第309医院器官移植研究室,北京,100091
- 折叠
摘要
Abstract
Objective To explore cytomegalovirus(CMV) infection in renal transplant recipients of the body lymphocyte sub sets expression and significance. Methods From January 2010 to December 2011 cytomegalovirus antigen positive renal transplant recipients (n=50) ,according to the virus and bacteria fungi infection status was divided into pure CMV infection group (n=13),intercurrent infection group (n=37) ,control group for renal normal renal transplants (n=10).The HCMV pp65 antigen tested via immune fluorescence assay,lymphocyte subsets of the samples was detectedby flow cytometry. Results The CD3+ lymphocyte in the CMV infection group and intercurrent infection group were (68. 6 + 8. 64)% and (61 ± 21. 74) % respectively. There were no significant differences compared with renal transplantation stable group (P>0. 05). The CD4+ lymphocyte of them were (23. 8 ± 0. 75) % and (21. 85 ± 8. 83) % respectively,and the difference had statistical significance compared with renal transplantation stable group (t=8. 232 8,8. 873 2, P<0. 05). The CD8+ lymphocyte in the CMV infection group and intercurrent infection group were (39. 2 ± 1. 53) % and (36. 2 ± 15. 1) % respectively,and the difference had statistical significance compared with renal transplantation stable group (t=7. 660 5 ,3. 23 5, P<0. 05). The CD4 + /CD8+ of them were (0. 63 ± 0. 11) and (0. 69 ± 0. 18) respectively, and the difference had statistical significance (t = 5.988 5,8.396 9,P>0.05). The B cell in the CMV infection group and intercurrent infection group were (10.2 + 3.43)% and (11.8 ± 20. 9')% respectively,and there were no significant differences compared with renal transplantation sta ble group (P<0. 05). The NK of them were (8. 01 ± 2. 5) % and (7. 2 ± 10. 14) % respectively, and renaltransplantation stable group were significantiy lower than,their difference was statistically significant (t=2. 850 4,2. 363 8, P>0. 05). Conclusion By flow cytometry analyzer to measure lymphocyte subsets and dynamic monitoring the change,for early diagnosis and differential diagnosis of severe CMV infection and the reasonable application of immune inhibitors have certain guiding. 关键词
肾移植/巨细胞病毒感染/淋巴细胞亚群Key words
renal transplant/cytomegalovirus infection/ lymphocyte subsets分类
医药卫生引用本文复制引用
高钰,肖漓,石炳毅,冯凯,马锡慧,孔祥瑞..淋巴细胞亚群在肾移植术后巨细胞病毒感染患者中的表达及意义[J].现代检验医学杂志,2013,28(1):21-23,3.
