现代检验医学杂志2013，Vol.28Issue(1)：106-108,3.DOI:10.3969/j.issn.1671-7414.2013.01.035
乙型肝炎病毒表面抗原ELISA实验C50浓度的确定及意义
Determination and Significance of C50 Concentration of Hepatitis B Virus Surface Antigen ELISA Test
陈瀑1
作者信息
- 1. 重庆医科大学附属第一医院检验科,重庆400016
- 折叠
摘要
Abstract
Objective To get the gray area of hepatitis B virus surface antigen (HBsAg) by determination C50 concentration HBsAg of ELISA,and compare with two kinds of testing reagents of HBsAg. Methods According to the definition of criti cal point of qualitative test,critical point concentration (C50) of HBsAg,C5 concentration and C95 concentration of HBsAg were determined with a series of different concentration samples using two kinds of HBsAg regents by enzyme linked immu nosorbent assay;and gray area of two kinds of HBsAg reagents was gained respectively. Results C50 concentration of A re agent was 0. 40 IU/ml and S/CO value of gray area was 0. 84 ~ 1. 19. C50 concentration of B reagent was 0. 35 IU/ml and S/ CO value of gray area was 0. 72 ~ 1. 26. Conclusion Serum concentration of C50 concentration of two kinds of HBsAg rea gent was different,likewise the gray area was different. Reagent and method should be evaluated in Laboratory. C50 concen tration and gray area of HBsAg should be set up.关键词
酶联免疫吸附试验/乙型肝炎病毒表面抗原/C50/灰区Key words
ELISA/ HBsAg/C50 concentration/gray area分类
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陈瀑..乙型肝炎病毒表面抗原ELISA实验C50浓度的确定及意义[J].现代检验医学杂志,2013,28(1):106-108,3.
