陕西医学杂志2013，Vol.42Issue(3)：263-265,封3,4.
三种不同剂量二乙基亚硝胺诱癌效果比较
The comparation of hepatocellular carcinoma model induced by three different concentration of diethylnitrosamine in rats
摘要
Abstract
Objective:To observe the effects of different concentration of diethylnitrosamine (DEN) in duced hepatocellular carcinoma model in rats, determine appropriate DEN dose to induced cancer, and ensure the post-experimental research. Methods: SPF male Wistar rats 96, weighing 150to 200g, were randomly divided into 4 groups(A, B,C,D). Groups A, B, C drinking water contained 40, 64, 80 ppm DEN for 16 weeks. The control drank the routine table- water. After 16 weeks, every group was sacrificed 12 rats, liver tissue, as well as observa-tion of liver cancer cases morphological examination and pathology. The remaining 12 rats in each group were to be observed the survival periods. Results: After 16w, there were no rats died of cancer. Pathological examination con-firmed that at 16th week, group A and D caught cancer was 0, group B induced cancer rate was of 83. 33%, C groups cancer rate was of 91. 16％, no significant difference in cancer rate between two groups. Group C showeds a bulky liver cancer, group A showed inflammation, edema, punctate necrosis , mild connective tissue proliferation, fatty degeneration. The liver surface of the group B showed diffuse focal areas of a typical hyperplasia of liver cells, peritu moral connective tissue proliferation, did not find the performance of typical cirrhosis,the non-cancerous liver tissue of the group C showed deformation diffused edema, dotty-necrosis,per portal, inflammatory infiltration, con-nective tissue hyperplasia, lobular structure of most of the damage. DEN induced hepatocellu-lar carcinoma was HCC,rat hepatocellular carcinogenesis process roughly after liver cell injury, liver cell hyperplasia, hardening period and hepatocellular carcinogenesis period of three phrases. Similar to human liver cancer with the process and patho-logical changes characteristic lesions. Conclusion: Both of the concentration of 64ppm,80ppm DEN can be used as in-duced cancer dose,64ppm induced in cancer survival maybe more effectiveness than 80ppm. 64ppm concentration in-duced cancer is an ideal for the evaluation of drug efficacy in rat liver cancer model.关键词
肝肿瘤,实验性/二乙基亚硝胺/模型,动物/大鼠Key words
Liver neoplasms, Experimental / Diethylnitrosamine / Models,Animal / Rats分类
医药卫生引用本文复制引用
黄峰,韦艾凌..三种不同剂量二乙基亚硝胺诱癌效果比较[J].陕西医学杂志,2013,42(3):263-265,封3,4.基金项目
国家自然科学基金资助项目(30960475) （30960475）
