实用医学杂志2012，Vol.28Issue(9)：1438-1440,3.DOI:10.3969/j.issn.1006-5725.2012.09.017
缝隙连接蛋白Cx32与Cx43在癫痫发病中的作用
The roles of connexin 32 and connexin 43 play in the pathogenesis of epilepsy
摘要
Abstract
Objective To explore the relationship between gap junctions (GJ) formed by connexins (Cx) and epilepsy. Methods The expressions of Cx32 and Cx43 at different time-points in different brain regions were measured by immunohistochemistry in the epileptic rats induced by lithium chloride-pilocarpine. Results As compared to those in control group, the immunopositive expressions of Cx32 and Cx43 in hippocampus and cortex of epileptic rats were increased at 1h after epileptic seizures (Cx32 in hippocampus and cortex were 16.62 ± 4.51 and 14.85 ± 3.30, P 〈 0.05; Cx43 in hippocampus and cortex were 18.26 ± 4.03 and 18.65 ± 4.51, P 〈 0.01) , reached peak at 24 h (Cx32 in hippocampus and cortex were 46.53 ± 9.47 and 28.25 ± 8.69, P 〈 0.01; Cx43 in hippocampus and cortex were 39.77 ± 7.79 and 26.50 ± 6.56, P 〈 0.01), and then were gradually declined. Until 14 d, the expressions of Cx32 in hippocampus and cortex were significantly higher than those in control group (22.45 ± 6.56 and 15.92 ±3.16, P 〈 0.05), aiid the expression of Cx43 in hippocampus was still higher than that in control group (17.54 ± 3.77, P 〈 0.01), as well as that in cortex was decreased to normal levels. At 24 h, both of the expressions of Cx32 and Cx43 in hippocampus were significantly higher than those in cortex at the same time-point (P 〈 0.05). Conclusions Cx32 and Cx43 might be involved in the occurrence and development of epilepsy. GJ between neurons or astrocytes in the brain might be closely related to the pathogenesis of epilepsy.关键词
癫痫/缝隙连接蛋白/大鼠Key words
Epilepsy/ Connexin/ Rat引用本文复制引用
曾杨滨,刘宇明..缝隙连接蛋白Cx32与Cx43在癫痫发病中的作用[J].实用医学杂志,2012,28(9):1438-1440,3.基金项目
广东省医学科研基金资助项目(编号:A2008290) （编号:A2008290）
