实用医学杂志2012，Vol.28Issue(21)：3524-3527,4.DOI:10.3969/j.issn.1006-5725.2012.21.010
脑摄取平衡时丙泊酚对犬脑不同区域兴奋性氨基酸转运体2 mRNA的影响
Effect of propofol at uptake equilibrium on excitatory amino acid transporter-2 mRNA expression in different brain regions in dogs
摘要
Abstract
Objective To investigate the effect of propofol at uptake quilibrium on excitatory amino acid transporter-2 (EAAT2) mRNA expression in different brain regions in dogs. Methods Eighteen healthy male and female mongrel dogs, aged 12 - 18 months and weighed 10 ~ 12 kg, were divided randomly into high dose group (group H), low dose group (group L) and control group (group C), each group with 6 dogs. Dogs in group C and group H were anesthetized with propofol at a single bolus (5.5 and 7.0 mg/kg, respectively) in 15 s followed by propofol infusion at a constant rate [55 and 70 mg/(kg·h), respectively]. Then, blood samples (2 mL) were taken from the internal carotid artery and jugular vein to measure plasma propofol concentration using high-performance liquid chromatography ultra-violet spectroscopy (HPLC-UV). Each dog was sacrificed by intravenous injection of 10% KC1 2 mg/kg. The hypothalamus, subthalamus, dorsal thalamus, hippocampus, pons, parietal lobe and frontal lobe were obtained and used to determine EAAT2 mRNA expression by Quantitative Real-time PCR (qRT-PCR). Results When propofol infusion at a constant rate for 50 min, no significant differences were found between plasma propofol concentration in internal carotid artery and jugular vein in both group L and group H (P > 0.05). EAAT2 mRNA levels in group L and group H were higher than that in group C (P < 0.05) in hypothalamus, hippocampus and parietal lobe, with no significant differences between group L and group H (P > 0.05). No significant differences of EAAT2 mRNA expression were found among the three groups in other brain regions (P > 0.05). Conclusions Brain uptake of propofol reached equilibrium at 50 minutes after propofol infusion at a constant rate. Propofol could increase EAAT2 mRNA expression significantly at this time point in hypothalamus, hippocampus and parietal lobe, without no significant doses-dependent effects.关键词
二异丙酚/兴奋性氨基酸转运体2/脑/静脉麻醉Key words
Propofol/Excitatory amino acid transporter-2/Brain/ Intravenous anesthesia引用本文复制引用
李洋,林春水,古妙宁,杨晶晶,范钦,陈莺..脑摄取平衡时丙泊酚对犬脑不同区域兴奋性氨基酸转运体2 mRNA的影响[J].实用医学杂志,2012,28(21):3524-3527,4.基金项目
广东省自然科学基金资助项目(编号:91510501000054,10151051501000104) （编号:91510501000054,10151051501000104）
