实用医学杂志2013，Vol.29Issue(1)：26-28,3.DOI:10.3969/j.issn.1006-5725.2013.01.014
JNK/MAPK信号通路在咖啡酸诱导人白血病K562细胞凋亡中的作用
Role of JNK/MAPK in caffeic acid-induced apoptosis of leukemia K562 cells
莫清华 1殷清华2
作者信息
- 1. 421001,湖南省衡阳市第-人民医院
- 2. 414000,湖南省岳阳市二人民医院
- 折叠
摘要
Abstract
Objective To determine the role of JNK/MAPK signal transduction pathways in caffeic acid-induced apoptosis in leukemia K562 cells. Methods K562 cells growth inhibition was measured by MTT assay. Apoptosis of K562 cells was studied by AO/EB fluorescence stain and flow cytometry with PI staining. Expression of JNK, phosphorylation of JNK (p-JNK) MAPK were determined by Western blot analysis. Results Caffeic acid could induce apoptosis of K562 cells and the activity of p-JNK in K562 cells was apparently increased. However, after treatment with JNK/MAPK inhibitor (SP600125), the inhibitory effect of caffeic acid on K562 cells was significantly weakened, the apoptotic rate of the cells and the activity of p-JNK also decreased dramatically. Conclusions Caffeic acid can induce apoptosis of K562 cells, and JNK inhibitor can significantly inhibit the apoptosis induced by caffeic acid in K562 cells. Caffeic acid induced apoptosis of K562 cells involves the activation of JNK/MAPK.关键词
白血病/咖啡酸/人白血病K562细胞/细胞凋亡/JNK/MAPKKey words
Leukemia/Caffeic acid/Leukemia K562 cells/Apoptosis/JNK/MAPK引用本文复制引用
莫清华,殷清华..JNK/MAPK信号通路在咖啡酸诱导人白血病K562细胞凋亡中的作用[J].实用医学杂志,2013,29(1):26-28,3.
