实用肿瘤杂志2012，Vol.27Issue(6)：583-586,4.
MicroRNA-181a提高A549/DDP细胞顺铂敏感性的研究
MicroRNA-181a enhances the sensitivity of A549/DDP cells to cisplatin via inhibiting Bcl-2 expression
摘要
Abstract
Objective To investigate the effect of miR-181a on the sensitivity of A549/DDP cells to eisplatin and its mechanism. Methods A549/DDP cells were transfected with miR-181a mimic. The cell growth was detected by MTT method. The expression of microRNA-181a was measured by fluorescence quantitative polymerase chain reaction. Bcl-2 protein level was determined by Western blotting. The apoptosis of cells was measured by Annexin V/PI methods. Results miR-181a significantly enhanced the sensitivity of A549/DDP cells to eisplatin and the apoptosis induced by eisplatin. After transfected with microRNA-181a mimic, Bcl-2 protein level decreased in A549/DDP cells. Conclusions MicroRNA-181a can enhance the sensitivity of A549/DDP cells to eisplatin, which may be associated with down-regulation of Bcl-2 expression.关键词
肺肿瘤/微RNAs/腺癌/顺铂/药理学/转染/细胞凋亡Key words
lung neoplasms/ microRNAs/ adenocarcinoma/ cisplatin/pharmacology/ transfection/ apoptosis分类
医药卫生引用本文复制引用
冯春来,王智刚,李皓..MicroRNA-181a提高A549/DDP细胞顺铂敏感性的研究[J].实用肿瘤杂志,2012,27(6):583-586,4.基金项目
常州市青年医学创新人才计划(常卫科教[2010]368号) （常卫科教[2010]368号）
