生殖医学杂志2012，Vol.21Issue(z1)：77-86,10.DOI:10.3969/j.issn.1004-3845.2012.z1.014
Expression of HIF-1α, TGF-β1, Muc1 and MMP-9 in placenta: preliminary pathophysiological study of preeclampsia
Expression of HIF-1α, TGF-β1, Muc1 and MMP-9 in placenta: preliminary pathophysiological study of preeclampsia
摘要
Abstract
Objective: To investigate expressions of hypoxia-inducible factors-1α (HIF-1α),transforming growth factor-β1 (TGF-β1),mucinl (Mucl) and matrix metalloproteinase-9 (MMP-9) in the placenta collected from the preeclampsia patients and normal pregnant women,so as to explore the possible pathophysiological mechanism of preeclampsia.Methods: The placenta villus tissues were obtained from 35 preeclampsia women,including 16 mild preeclampsia and 19 severe preeclampsia,and 20 normal pregnant women,within 5 minutes after placental expulsion.Expressions of HIF-1α,TGF-β1,Mucl and MMP-9 were detected by Western blot.Cellular location was observed by immunohistochemistry.Results: (1) HIF-la was mainly located in cytoplasm and nucleus of placental villous syncytiotrophoblast.Expression level of HIF-1α in the severe preeclampsia group was significantly higher than that in the mild group or control group (P＜0.01).(2) TGF-β1 was located in the trophoblast cell and exuviates membrane.Expression level of TGF-β1 in the severe and mild preeclampsia groups was significantly higher than that in control group (P＜0.05).(3) Mucl was located in trophoblast cell and exuviates membrane.Expression level of MUC1 in the severe preeclampsia group was significantly higher than that in the mild preeclampsia group and control group (P＜0.01).(4) MMP-9 was located in the trophoblast cell and villous stroma,exuviates membrane.Expression levels of MMP-9 in the two preeclampsia groups were lower than that in control group (P＞0.05).Conclusion: Expressions of HIF-1a,TGF-β1 and Mucl increased in the placenta of preeclampsia group,while MMP-9 decreased.Mucl can be induced and regulated by HIF-1α and TGF-β1.Over-expression of Mucl in placenta can significantly suppress the activation of MMP-9,which may influence the infiltration of trophoblast cells.The increased expression of HIF-1α and TGF-β1 in placenta may induce higher level of Mucl.This study helped us to understand the pathophysiological mechanism of preeclampsia.关键词
Preeclampsia/ HIF-1α/ TGF-β1/ Muc1/ MMP-9Key words
Preeclampsia/ HIF-1α/ TGF-β1/ Muc1/ MMP-9引用本文复制引用
Lyu Yan-guan,Dai Xiao-nan,Liu Shan,Wei Hong,Gao Chao,Gao Li,Liu Jia-yin,Cui Yu-gui..Expression of HIF-1α, TGF-β1, Muc1 and MMP-9 in placenta: preliminary pathophysiological study of preeclampsia[J].生殖医学杂志,2012,21(z1):77-86,10.基金项目
This work is supported by projects from National Foundation of Science (81170559) and China 973 Program (2012CB944703).We gratefully acknowledge two Departments of Obstetrics in HuaiAn Women & Children's Hospital and First Affiliated Hospital of Nanjing Medical University for their help in the collection of placenta samples. （81170559）
