天津中医药2013，Vol.30Issue(1)：25-27,3.DOI:10.11656/j.issn.1672-1519.2013.01.09
火针干预脊髓损伤大鼠后血清对神经干细胞增殖分化的影响
Influence of the serum of SCI rats after fire needle intervention on the proliferation and differentiation of neural stem cells
李岩 1周震 2程素利 3焦召华 3陈爽3
作者信息
- 1. 天津市公安医院,天津300042
- 2. 天津中医药大学第二附属医院,天津300150
- 3. 天津中医药大学,天津300193
- 折叠
摘要
Abstract
[Objective] To study the influence of the extracted serum of SCI rats after fire needle intervention on the proliferation and differentiation of neural stem cells in vitro. [Methods] Fifteen SD rats were randomly divided into sham operation, model and fire-needle groups, and the serum was extracted in each group after 3 days' corresponding intervention. The neural stem cells were separated from the spinal cord tissue in Kunming mice embryo after 14 days' pregnancy, and then cultured by all the above serum in each group to observe the proliferation and differentiation states of neural stem cells 7 days later. [Results] Neural stem cells of each group could survive, proliferate and differentiate into neurons. The proliferation number of neural stem cells in fire needle group was obviously more than that in sham operation and model group, and the percentage of differentiation into neurons in fire group was higher than that in the other groups (P<0.05). [Conclusions] The serum of SCI rats after fire needle intervention could promote the proliferation and differentiation into neurons of neural stem cells.关键词
脊髓损伤/火针/针刺/神经干细胞/增殖分化Key words
spinal cord injury/ fire needle/acupuncture/ neural stem cell/ proliferation and differentiation分类
医药卫生引用本文复制引用
李岩,周震,程素利,焦召华,陈爽..火针干预脊髓损伤大鼠后血清对神经干细胞增殖分化的影响[J].天津中医药,2013,30(1):25-27,3.
