天然产物研究与开发2012，Vol.24Issue(12)：1772-1776,5.
一株蓝麻黄内生真菌XJEG-GF-82的筛选、鉴定及其抑菌活性研究
Screening and Identification of XJEG-GF-82 ,An Antagonistic Endofungi from Ephedra.glauca and Its Antagonistic Activity Study
摘要
Abstract
XJEG-GF-82,an endophytic fungus,with broad antifungal and antibacterial activity to common crop and animal pathogens,was isolated from the endophytes of E. glauca using agar diffusion method in this study. The diameters of its inhibition zone against Bipolaris maydis, Fusarium oxysporumf. sp. niveum, Escherichia coli, Bacillus subtilis and Staphyloccocus aureus were determined to be 18 mm,16 mm,19 mm,25 mm and 27 mm respectively. XJEG-GF-82 was finally identified as Aspergillus ochraceus according to the morphological properties and D1 D2 regional sequence. The metabolites of XJEG-GF-82 were detected to contain polysaccharides and alkaloids, and the total concentration of poly-saccharides reached 160. 233 μg/mL after medium optimization. XJEG-GF-82 has provided a potential microbial resource for developing new chemical and natural anti-pathogen agents.关键词
拮抗内生真菌/系统鉴定/代谢物分析Key words
antagonistic endofungi/molecular identification/metabolites analysis分类
农业科技引用本文复制引用
阿布都卡地尔·阿布力孜,阿依乃再·孜亚克,吾甫尔·米吉提,阿布都沙拉木·阿布力克木..一株蓝麻黄内生真菌XJEG-GF-82的筛选、鉴定及其抑菌活性研究[J].天然产物研究与开发,2012,24(12):1772-1776,5.基金项目
国家自然科学基金项目(31160015) （31160015）
