武汉工程大学学报2012，Vol.34Issue(11)：40-43,4.DOI:10.3969/j.issn.1674-2869.2012.11.009
茚三酮法定性定量检测丝氨酸
Qualitative and quantitative determination of serine by ninhydrine
摘要
Abstract
An improved ninhydrine colorimetric method was established based on amino acid paper chromatography to detect serine in amino acid hydrolysate rapidly and simply. The method was applied to detect amino acids which were hard to separate. Firstly, ninhydrine was added to the developing agents, and the mixture of amino acids was undertaken chromatography on the filter paper. Chromatography ninhydrine and amino acid produced chromogenic reaction through which serine could be identified. Then, the different doses of serine standard solution were handled in the same way, a absorption-serine concentration standard curve was plotted by using serine standard solution to calculate the content of serine in the hydrolysate. The results show that the method possesses good repeatability, high precision and convenient operation under the best condition that pH of the solution is 5, the chromogenic agent is each 10 mL developing agent by adding 2. 5 mL 0. 1% ninhydrine and the heating time is 5 min under the temperature of 105 ℃.关键词
丝氨酸/茚三酮/显色法/含量测定Key words
serine/ninhydrine/chromatography/content detection分类
化学化工引用本文复制引用
关洪亮,熊倩,余训民,李庆新..茚三酮法定性定量检测丝氨酸[J].武汉工程大学学报,2012,34(11):40-43,4.基金项目
武汉工程大学科学研究基金项目(13102051) （13102051）
