摘要
Abstract
Objective To study the influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Esche-richia coli sepsis. Methods Canine models of Escherichia coli sepsis were established by infusing the live Escherichia coli ( 0111: B4) via vein. Eighteen hybrid dogs were anesthetized intravenously and randomly divided into two groups: the filtration group and the control group. The filtration group dogs underwent venous leukocyte depletion. T0,was set at the time of beginning infusion of the live Escherichia coli (0111: B4) , To was set at the time of beginning venous leukocyte depletion. Mean arterial pressure, pulse, body temperature, red blood cell concentration, platelet concentration, leukocyte concentration, TNF - α concentration, IL - 6 concentration in serum, CD11/ 18 expression, and CD14 expression in both groups were measured at To, To, T1 and T5. Results There were no significant differences in pulse, red blood cell concentration, platelet concentration, TNF - α concentration, and IL - 6 concentration between the two groups. Mean arterial pressure at T1 in the filtration group was greater than that in the control group ( P < 0. 05 ). The body temperature and leukocyte concentration at T1 in the filtration group was greater than that in the control group ( P < 0. 05 ). CD11/18 expression of neutrophils at T5 in the filtration group was lower than that in the control group ( P < 0. 05 ). CD14 expression of monocytes at T, , T5 in the filtration group was lower than that in the control group (P <0. 05). Conclusions Venous leukocyte depletion improves canine hemodynamics,
depresses leukocyte concentration, depresses the expression of the CD11/18 of neutrophils, CD14 of monocytes, whereas does not affect the inflammatory factor in serum.关键词
静脉血白细胞过滤/脓毒症/CD11/18/CD14Key words
venous leukocyte depletion/sepsis/CD11/ 18/ CD14分类
医药卫生
