西安交通大学学报（医学版）2013，Vol.34Issue(1)：11-15,5.
miR-195表达载体构建及其对肝癌细胞株HepG2植瘤抑制效果的影响
Construction of miR-195 expression vector and anti-tumorigenic effect of miR-195 on human hepatoma cell strain HepG2
摘要
Abstract
Objective To investigate the anti-tumorigenic effect of miR-195 on human hepatoma cell strain HepG2. Methods We constructed miR-195 expression vector by molecular cloning technique, transfected it into HepG2 cell strain by liposome, and then constructed miR-195 overexpression HepG2 cell strain by clone selection. Furthermore, we detected the tumorigenic potential of each different cell strain in vivo in nude mice. Results We successfully constructed miR-195 overexpression vector by analyzing both of the sequencing results and the fluorescent results. Furthermore, real time PCR results revealed that miR-195 expression level in miR-195 overexpression HepG2 cell strain was 2 000 as high as that of the related non-transfection HepG2 cells (P<0.01). Moreover, miR-195 overexpression cells exhibited less tumorigenic potential than that of the non-transfected groups. Conclusion miR-195 may act as a tumor suppressor in hepatoma development, suggesting an effective target in hepatorna therapy.关键词
miR-195/HepG2细胞株/动物模型/分子克隆技术/转染/肝癌/基因治疗Key words
miR-195/ HepG2 cell strain/ animal model/ molecular cloning technique/ transfection/ hepatocel-lular carcinoma/ gene therapy分类
生物科学引用本文复制引用
胡晓岩,姚嘉宜,李梦鹤,倪磊,王爱英,黄辰,宋土生..miR-195表达载体构建及其对肝癌细胞株HepG2植瘤抑制效果的影响[J].西安交通大学学报（医学版）,2013,34(1):11-15,5.基金项目
陕西省科技攻关项目重大科技专项(No.2010ZDKG-50) （No.2010ZDKG-50）
