西安交通大学学报（医学版）2013，Vol.34Issue(1)：20-23,49,5.
新型纳米金荧光双标survivin反义寡核苷酸探针的合成及其对人恶性黑素瘤A375细胞的影响
Synthesis of the probe of survivin antisens oligonucleotide modified gold nanoparticles and its impact on A375 cells
摘要
Abstract
Objective To synthesize the probe of survivin antisense oligonucleotide modified gold nanoparticles by making use of the biological and optical characteristics of gold nanoparticles and to analyze its impact on A375 cells. Methods Citrate-stabilized gold nanoparticles were prepared using published procedures. Thiol-modified oligonucleotides were added to gold colloids to make nano-probes. Then the complementary reporter sequence was combined to prepare "nano-flare" probes. A375 cells were grown with media containing nano-flares probes. Fluorescent images were observed under the fluorescence microscope. Cell proliferation was measured by MTT and cell apoptosis was detected by Annexin V/PI double staining method. Results The nano-probes could inhibit A375 cell proliferation significantly (P<0.05) and induce cell apoptosis. Conclusion The nano-probes that can be stably synthesized can inhibit proliferation of A375 cells and induce their apoptosis.关键词
纳米金/反义寡核苷酸/survivin/A375细胞Key words
gold nanoparticle/ antisense oligonucleotide/ survivine/ A375 cell分类
医药卫生引用本文复制引用
李萍,王梅,肖生祥,王冰,霍佳..新型纳米金荧光双标survivin反义寡核苷酸探针的合成及其对人恶性黑素瘤A375细胞的影响[J].西安交通大学学报（医学版）,2013,34(1):20-23,49,5.基金项目
国家自然科学基金资助项目(No.30872410) （No.30872410）
