西安交通大学学报（医学版）2013，Vol.34Issue(1)：38-41,4.
胰岛素产生细胞的诱导分化及微囊对其分泌能力的影响
Effects of induced differentiation and micro-capsule on the secretion capacity of insulin-producing cells
摘要
Abstract
Objective To study the effects of micro-capsule on the secretion capacity of insulin-producing cells (IPCs). Methods Mouse bone marrow mesenchymal stem cells (BMMSCs) were isolated, induced and purified. Rat pancreatic extract (RPE) was extracted from rats' pancreases. BMMSCs were induced by RPE. The induced cells were randomly divided into micro-encapsulated group and non-micro-encapsulated group. The experiment of glucose stimulation was performed to detect the level of insulin and C peptide release, respectively, at different time points (1 week, 2 week, 3 week, 4 week and 5 week). Results The experiment of glucose stimulation showed that the level of insulin secretion did not differ in micro-encapsulated IPCs and non-micro-encapsulated IPCs at 1 week. The levels of insulin and C peptide secretion started to decline in non-micro-encapsulated IPCs at 2 week, while those did not decline in micro-encapsulated IPCs until 4 week. Conclusion The micro-capsule can extend the effect duration of IPCs, which supports the function of IPCs.关键词
微囊/胰岛素产生细胞/骨髓间充质干细胞/诱导分化Key words
micro-capsule/ insulin-producing cells (IPCs)/ bone marrow mesenchymal stem cells (BMMSCs)/ induced differentiation分类
医药卫生引用本文复制引用
王雅光,田鹤,刘建生,穆长征..胰岛素产生细胞的诱导分化及微囊对其分泌能力的影响[J].西安交通大学学报（医学版）,2013,34(1):38-41,4.基金项目
辽宁省自然科学基金资助项目(No.201102138) （No.201102138）
