摘要
Abstract
Objective To detect sputum samples from patients with stable chronic obstructive pulmonary disease (COPD) and acute excerbations of chronic obstructive pulmonary disease (AECOPD) for bacteriological analysis so as to explore the relationship of bacterial infection with COPD incidence and progress. Methods We employed specifc primers of seven common pathogenic bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa , Streptococcus pneumoniae , Staphylococcus aureus, Haemophilus influenzae, Moraxella catarrhalis and Acinetobacter baumannii) in COPD to detect the sputum samples from 42 patients with stable COPD and 66 patients with AECOPD by using polymerase chain reaction (PCR) method. Then we compared them with the sputum samples from 11 normal controls to screen the infection of these pathogens. Results Klebsiella pneumoniae was 8/42 in stable COPD (19%), 4/66 in AECOPD (6.1%), and was not detected in normal controls. Pseudomonas aeruginosa was 2/42 in stable COPD (4.8%), 11/66 in AECOPD (16. 7%), and was not detected in normal controls. Streptococcus pneumoniae was 42/42 in stable COPD (100%), 26/66 in AECOPD (39.4%), and 11/11 in normal controls. Staphylococcus aureus was not detected in stable COPD or normal controls, and was 6/66 in AECOPD (9.1%). Haemophilus influenzae was 39/42 in stable COPD (92.9%), 51/66 in AECOPD (77.3%), and 11/11 in normal controls. Moraxella catarrhalis was 12/42 in stable COPD (28. 6%), 6/66 in AECOPD (9.1%), and was not detected in normal controls. Acinetobacter baumannii was 3/42 in stable COPD (7.1%), 5/66 in AECOPD (7.6%),and was not detected in normal controls. The detection rates of Klebsiella pneumoniae, Streptococcus pneumoniae. Staphylococcus aureus. Haemophilus influenzae, and Momxella catarrhalis differed significantly between stable COPD and AECOPD groups (P<0. 05); those of Pseudomonas aeruginosa and Acinetobacter baumannii did not differ significantly between the two groups (P>0.05). The sequencing results were consistent with the detection results. Conclusion The flora level in the upper respiratory tract of patients with stable COPD is close to that in normal patients, while that in AECOPD patients is significantly reduced. Infections with certain bacteria are a major cause of AECOPD onset, but not all bacterial infections are associated with the progression of COPD.
关键词
慢性阻塞性肺疾病/聚合酶链式反应/痰/常见致病菌/细菌感染/呼吸道菌群/细菌学分析Key words
chronic obstructive pulmonary disease (COPD)/ polymerase chain reaction/ sputum/ common pathogen/ bacterial infection/ respiratory flora/ bacteriological analysis分类
医药卫生
