西安交通大学学报（医学版）2013，Vol.34Issue(1)：118-121,4.
不同冷冻方法对兔颈总动脉形态结构及活性的影响
Effects of different freezing methods on morphology and viability of rabbit carotids
师桃 1吕毅 2闫炀 1耿希刚1
作者信息
- 1. 西安交通大学医学院第一附属医院心血管外科,陕西西安 710061
- 2. 西安交通大学医学院第一附属医院肝胆外科,陕西西安 710061
- 折叠
摘要
Abstract
Objective To assess the effects of vitrification and slow freezing method on the morphology and viability of rabbit carotids. Methods Rabbit carotids were harvested and cryopreserved by vitrification and slow freezing method for 2 weeks, and then thawed slowly in water bath at 37 ℃ . The fresh carotids were taken as controls. The morphological changes of all carotids were observed with the naked eye, optical microscope and electron microscope. The fresh and frozen-thawed arteries were cultured in vitro. Viability, regeneration and proliferation of the cells were observed and described by cellular growth curve. Results The morphological studies demonstrated that some of endothelium cells (ECs) fell off, some of the smooth muscle cells (SMCs) and fibroblast cells swelled or denaturalized after freeze-thawing procedure. Vitrification, however, was less detrimental to vessel structure than slow freezing method. The cells of vitrified carotids and frozen carotids grew out from tissues on the 6th and 7th day. The biological characteristics were similar to those of the fresh carotids. The growth curve showed that cell viability and proliferation were preserved perfectly in vitrification group compared to slow freezing group, but inferior to the fresh carotids. The cells cultured from all carotids were identified through immunohistochemical staining and proved to be SMCs. Conclusion Vitrification does less damage to the morphology and viability of vessels, so rabbit carotids preserved by vitrification are better than those preserved by slow freezing method.关键词
颈总动脉/玻璃化法/慢速冷冻/深低温保存/细胞活力/增殖Key words
rabbit carotid/ vitrification/ slow freezing/ cryopreservation/ cell viability/ proliferation分类
医药卫生引用本文复制引用
师桃,吕毅,闫炀,耿希刚..不同冷冻方法对兔颈总动脉形态结构及活性的影响[J].西安交通大学学报（医学版）,2013,34(1):118-121,4.
