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栀子苷的定向分离提取方法建立

伏建峰 杜晖俐 史清海 周郁 刘正祥 冉继华 葛迪 李晓玲 何雁

西北国防医学杂志2013,Vol.34Issue(1):1-4,4.
西北国防医学杂志2013,Vol.34Issue(1):1-4,4.

栀子苷的定向分离提取方法建立

Directional isolation and extraction of jasminoidin from aqueous extract of Gardenia jasminoides Ellis

伏建峰 1杜晖俐 2史清海 1周郁 1刘正祥 1冉继华 1葛迪 1李晓玲 1何雁1

作者信息

  • 1. 兰州军区乌鲁木齐总医院全军临床检验诊断中心,新疆乌鲁木齐830000
  • 2. 新疆军区机关门诊部检验科
  • 折叠

摘要

Abstract

Objective; To establish the method of directional isolation and extraction of jasminoidin from aqueous extract of Gardenia jasminoides Ellis ( GjE). Methods: Based on the biosensor technology platform for screening anti - LPS compound which targeted as Lipid A, the aqueous extract of GjE was isolated and purified gradually by using macroporous adsorptive resins, membrane separation and HPLC technology in turn. Results:The jasminoidin was attained form aqueous extract of GjE, the value of specific binding to Lipid A was 112 arc/s. Conclusion; The established directional isolation mode of jasminoidin is more effective than that of traditional method, which is applicable for enriching and purifying anti - LPS active compound from natural medicine.

关键词

栀子/栀子苷/高效液相色谱/提取分离

Key words

Gardenia jasminoides Ellis/ Jasminoidin/ High performance liquid chromatography/ Isolation

分类

医药卫生

引用本文复制引用

伏建峰,杜晖俐,史清海,周郁,刘正祥,冉继华,葛迪,李晓玲,何雁..栀子苷的定向分离提取方法建立[J].西北国防医学杂志,2013,34(1):1-4,4.

基金项目

国家自然科学基金面上项目(81071535) (81071535)

西北国防医学杂志

OACSTPCD

1007-8622

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